1,3-Bis[tris(hydroxymethyl)methylamino]propane is used as a buffer for polymerase chain reaction and molecular biology. It is also used with hydrochloric acid buffer for stabilization of farnesyl diphosphate isolated from a strain of saccharomyces cerevisiae. It acts as a ligand and forms dinuclear hydroxo complexes with Lanthanides(III). Further, it is useful for calibration of genetically encoded pH indicators expressed in the cytosol or mitochondria. It is also employed to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin. In addition to this, it is used in the purification of glucose-binding protein from membranes of sulfolobus solfataricus and as a non-zwitterionic buffer, which is used in the pH range between 6.3 and 9.5.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.