ELISA Substrates and Reagents

TMB (3,3',5,5' tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 nm and 652 nm. The color then changes to yellow (measured at 450 nm) upon addition of sulfu

Thermo Scientific™ Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers. OPD (o-phenylenediamine dihydrochloride) yields a water-soluble yellow-orange pr

Thermo Scientific™ Pierce ABTS Substrates are tablets and a ready-to-use solution of a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods. ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-di

The CDP-Star™ Substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.This CDP-Star™ chemiluminescent substrat

Chemiluminescent substrates provide highly sensitive replacements for the widely used fluorogenic substrate methylumbelliferyl phosphate (MUP), and the colorimetric substrate p-nitrophenyl phosphate (pNPP). Low background luminescence coupled with high-i

This CSPD™ substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.This CSPD™ chemiluminescent substrate for a

When combined with DynaLight Trigger Solution, DynaLight Substrate with RapidGlow Enhancer delivers maximum signal within seconds. The environmentally-friendly DynaLight Trigger Solution provides maximum signal within 1 second of addition, with a longer

Reagent sold ready-to-use.

Buffer Kit for Antibody Pairs is for use with Invitrogen Antibody Pairs. Kit provides sufficient materials for ten ELISA plates.Build Your Own Immunoassay, Cell Analysis, Immunoassays

ELISA Carbonate Coating Buffer is intended for use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA.The buffer is a 50 mM carbonate buffer, pH 9.4.

ELISA Phosphate Coating Buffer is intended for use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA.The buffer is a 10 mM phosphate buffer, pH 7.4.

ELISA Stop Solution is a ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution. As TMB substrate solution reacts with horseradish peroxidase, it produces a pronounced bl

Type: Reagent Format: 30X Concentrate

The Galacton-Plus™ Substrate is a concentrated chemiluminescent substrate for E.coli β-galactosidase assays.For Research Use Only. Not for use in diagnostics procedures.

Galacton-Star substrate is a chemiluminescent substrate designed for the rapid, and sensitive detection of beta-galactosidase in cell lysates. The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric