Flow Cytometry Compensation, Counting and Calibration

The AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry. Species reactivity—for use with all isotypes of mouse antibody conjugates Maximum laser compatibility—for use with all lasers Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Often, however, cellular-based compensation controls are not completely effective, as many antigens are not highly expressed, and dimly stained cells can lead to inaccurate compensation settings. The AbC™ Anti-Mouse Bead Kit was developed to address this issue. How it works Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads). After mixing with a fluorophore-conjugated mouse antibody, the two components provide a disti

The AbC™ Anti-Rat/Hamster Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated to rat or hamster antibodies with any laser in flow cytometry. Species reactivity—for use with all isotypes of rat and hamster antibody conjugates Maximum laser compatibility—for use with all lasers Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Often, however, cellular-baseThe AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry. Species reactivity—for use with all isotypes of mouse antibody conjugates Maximum laser compatibility—for use with all lasers Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Often, however, cellular-base

Broadest multispecies reactivity—for use with all isotypes of mouse, rat, hamster, and rabbit antibody conjugatesBrightest signal—most sensitive antibody-capture beads availableMaximum laser compatibility—for use with all lasersEach kit provides polystyr

AccuCheck Counting Beads are an efficient single-platform method for absolute cell counts that combines the advantages of direct flow cytometric immunophenotyping with the use of two different fluorescent beads (A and B beads). These two fluorospheres ar

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5μm Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers are fluorescent-dye infused microspheres that

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0μm Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers are fluorescent-dye infused microspheres that

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5μm Alignflow™ Flow Cytometry Alignment Beads for Red Lasers are fluorescent-dye infused microspheres that a

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0μm Alignflow™ Flow Cytometry Alignment Beads for Red Lasers are fluorescent-dye infused microspheres that are excitable at 630–640 nm with emission in the range of 645–680 nm. View information for all flow cytometry calibration and size reference beads. AlignFlow™ beads provide: Uniformity—very low FSC CV and intensity CV Consistency in production, creating ideal reference standards Confidence through reliable, optimal instrument performance Compatibility—use with any instrument The AlignFlow™ beads are highly uniform with respect to size and fluorescence intensity, resulting in low FSC CV and intensity CV. The beads approximate the size, emission wavelength, and intensity of many biological samples and permit the calibration of the flow cytometer’s laser source, optics, and stream flow without wasting valuable and sensitive experiment

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5μm Alignflow™ Flow Cytometry Alignment Beads for UV Lasers are fluorescent-dye infused microspheres that ar

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0μm AlignFlow™ Flow Cytometry Alignment Beads for UV Lasers are fluorescent-dye infused microspheres that ar

ArC™ Amine Reactive Compensation Bead Kit is a bead-based compensation tool specifically optimized for use with LIVE/DEAD™ Fixable dead cell stain kits. Ready-to-use control Eliminates the hassle of heat-treating cells as a control, saving precious sampl

The Bacteria Counting Kit accurately enumerates bacteria by flow cytometry. The kit includes the SYTO™ BC dye, which is also available separately (Cat No. S34855), a high-affinity nucleic acid stain that easily penetrates both gram-negative and gram-posi

BrightComp eBeads compensation beads provide a consistent, accurate, and simple-to-use technique for setting flow cytometry compensation when using fluorescent protein-expressing samples. Three levels of intensity, perfect for any samples no matter the l

Reliable standards for flow cytometry sorter instrument setup and calibration.Optimized for checking cell sorter settings such as drop delay and efficiency (cell loss during sorting).Available for use with UV, blue, green/yellow, and red lasers.For blue

Molecular Probes™ Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can b