Additional Cell Culture Reagents and Supplements

Laminin is the major glycoprotein component of basement membranes, and has a molecular weight of 850,000 daltons. This preparation of mouse Laminin was isolated from the Engelbreth-Holm-Swarm (EHS) sarcoma and is supplied at a concentration of 0.5-2.0 mg

Gibco N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein's N-1 formulation, containing optimized concentrations of insulin, iron-rich holo-transferrin, progesterone, putrescine, and selenite. N-2 Supplement is recommended

Sodium Pyruvate is commonly added to cell culture medium as an energy source in addition to glucose. Since the cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not necessary to supplement all cultures. However, if

Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G solution is now routinely used with other basal media to support a wide variety of cell types in the presence of 2 to 4% FBSInsulin promotes glucose and amino acid uptake, lipogenes

This original and genuine B-27 Supplement is a cGMP-manufactured, serum-free solution designed to ensure the consistent, reliable, and high-quality growth of embryonic, post-natal, and adult hippocampal neurons, as well as other CNS neurons. With a prove

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.For established protocols using B-27 Supplement, access our Gibco Neurobiology Pro

Gibco™ Water for Injection (WFI) for Cell Culture meets the USP monograph for Water for Injection packaged in bulk for commercial use elsewhereThe water is prepared by distillation and filtration (0.1 μm), with no added substancesIn addition to meeting U

This distilled water is high-quality water for use as a solvent in the preparation of cell culture media and laboratory reagents. Available in many sizes to meet your needs, this distilled water features:Cell culture qualityProven performanceSuitable for

Performed while counting cells with hemocytometer during routine subculturing, but can be performed any time cell viability needs to be determined quickly and accuratelyDye exclusion test is based upon concept that viable cells do not take up impermeable

Gibco™ KaryoMAX™ Colcemid™ Solution in PBS is a 10μg/ml N-desacetyl-N-methylocolchicine (Colcemid™) solution made in Phosphate Buffered Saline (PBS). It is used for arresting cells in metaphase so the chromosomes can be separated for cytogenetic studies

Gibco Poloxamer 188 is a non-ionic surfactant used to control shear forces in suspension cultures, reduce foaming in stirred cultures, and minimize cell attachment to glass surfaces. It is widely utilized to protect cells in suspension from damage during

CultureOne Supplement is a chemically-defined, serum-free supplement designed to significantly improve the differentiation of neural stem cells (NSCs) to neurons. Compared to conventional differentiation methods where NSCs can overgrow and become burdens

FoamAway™ Irradiated AOF is a ready-to-use antifoaming agent designed to offer high performance and convenience in combating foam in your cell culture system. It is prediluted, presterilized, and packaged to connect directly to your bioreactor with no fu

Gibco™ Glucose Solution contains D-glucose at a concentration of 200 g/L and is used as a serum-free medium supplement suitable for cell culture. Glucose Solution can be added to a variety of Gibco™ cell culture media including but not limited to Dulbecc

Pluronic F-127 *20% solution in DMSO* is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media. Pluronic F-127

Recombinant human Laminin 521 (rhLaminin-521) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8™ Medium, rhLaminin-521 has been proven to promote cellular survival under stressful conditions in the absence of small molecule inhibitors. rhLaminin-521 has been shown to support PSC growth for >20 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. rhLaminin-521 also provides optimum reprogramming efficiency of human somatic cells as well as human pluripotent stem cell survival during feeder-dependent to feeder-free transitions when used in conjunction with Essential 8 Medium. For a kit that combines the medium and substrate, see the Essential 8™ Adaptation Kit. rhLaminin-521 is: Robust—transition cells between feeder-dependent and feeder-free culture systems with minimal effort Efficient—easily re

Mercaptoethanol is not stable in solution. so most protocols require daily supplementationConcentration of 55mM in Dulbecco's Phosphate Buffered Saline (DPBS)Manufactured at a cGMP compliant facility, located in Grand Island, New York, registered with th

The 4% Agarose Gel melts at 65 to 70°C and remains fluid at 37°CWhen diluted with concentrated insect cell culture medium, Gibco™ 4% Agarose produces a gel firm enough to immobilize local infections, yet ductile enough to allow for the efficient removal

AlgiMatrix™ Dissolving Buffer can be used with any of the AlgiMatrix™ 3D Culture Systems to dissolve sponges. It is safe to use with all cell types and will not disrupt cell spheroids, clusters or other 3D structures. Can be used right in the well or spo

AlgiMatrix™ Firming Buffer is for use with the AlgiMatrix™ 3D Culture System to facilitate the formation of firm sponges in the 96 well and 24 well plate formats. This buffer allows the users to adjust the firmness of the sponge to meet specific cell and

Easy-to-use format: Gibco AmnioMAX C-100 Complete Medium is a two-part kit for preparation of a complete medium that requires no additional supplementation. The complete medium can be stored at 2-8°C for up to ten daysQuality and performance testingEvery

GIBCO™ Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that con

Formulated as 150 g Yeast Extract Powder (YEP) per liter of distilled waterProduced from ultra-filtered Baker's yeastManufactured at a cGMP compliant facility, located in Grand Island, New York, registered with the FDA as a medical device manufacturer an

The B-27™ Electrophysiology Kit contains Neurobasal™ Medium, Electro and B-27™ Supplement, Electro, both specifically developed to enhance network spike rates in cultured neurons when compared with other media and supplement combinations. Electrophysiology is the fundamental technique to assess such electrical activities to study the functions and dysfunctions in cultured neurons. Using the B-27™ Electrophysiology Kit in your neuronal studies offers: Increased synapse densities More spontaneous spike rates Improved multielectrode array efficiency More Synapses, More Signal, Simpler Testing The B-27™ Electrophysiology Kit is an improvement to Neurobasal™ and B-27™ combination for cultured networks. Hippocampal and cortical neurons cultured in this media system show an increased density of synapses and neurotransmitter receptors which produce higher spike rates in neural networks, helping to simplifying your study. Relevant Results for Your Electrophysiology Studies Using Invitrogen™ B-2

B-27 Plus Supplement is a serum-free supplement that provides the highest rate of in vitro survival of primary rodent and human stem cell-derived neurons when used with Neurobasal Plus Medium. B-27 Plus Supplement features an optimized formulation, upgra

The custom B-27™ Supplement (50X) is a serum-free additive for the growth and expansion of retinal ganglion cells. It is supplied as a 50X liquid concentrate and it is used with a basal medium, such as Neurobasal™, and other additives, such as GlutaMAX-1

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Antioxidants (AO) is a customized version of the original B-27 Supplement without five antioxidants (vitamin E, vitamin E acetate, superoxide dismutase, catalase, and g

Gibco B-27 Supplement minus vitamin A is a customized, serum-free formulation based on the original, most cited neuronal cell culture supplement, designed specifically to support the proliferation and cultivation of neural stem cells (NSCs) and neural pr

B-27 Supplement XenoFree is based on the classic B-27 Supplement formulation and contains no animal-derived components at the primary component level. Designed with the translational stem cell researcher in mind, B-27 Supplement XenoFree can be used to s

B-27 Supplement XenoFree Minus Insulin is based on the classic B-27 Supplement formulation and contains no animal-derived components at the primary component level. The absence of insulin makes this formulation ideal for the growth of hippocampal and oth

B-27 Supplement XenoFree Minus Vitamin A is based on the classic B-27 Supplement formulation and contains no animal-derived components at the primary component level. The absence of vitamin A (retinyl acetate) makes this formulation ideal for the cultiva

The BacMam Enhancer helps to increase expression of BacMam reagents in mammalian cells.To use, simply add the BacMam enhancer to cell culture media with or without serum that have already been treated with the BacMam reagent, then incubate cells and repl

The Cell Culture Contamination Detection Kit is a simple and effective procedure for screening tissue cultures for contamination by microorganisms. The kit not only detects contaminants, but it also identifies the contaminant type. Three fluorescent dyes

Gibco™ CHO CD EfficientFeed™ A liquid nutrient supplement is an animal origin-free, chemically defined complete feed formulation that provides up to 2-5 fold improvement in titers used in fed batch versus batch culture. It contains no proteins, hydrolysa

Collagen I, Rat Tail is the most common fibrillar collagen (90%) found in skin, bone, tendons, and other connective tissues. It is supplied at 3 mg/mL in a 20 mL vial providing the flexibility to dilute to lower concentrations. The detailed gelling proce

Cryopreserved Hepatocyte Plating Medium (CHPM®) used for plating Gibco cryopreserved human hepatocytes for optimal attachment and monolayer formation. For research use only. Not intended for human or animal therapeutic or diagnostic use.Related LinkLearn

Cryopreserved Hepatocyte Recovery Medium (CHRM®) is used for thawing Gibco cryopreserved human hepatocytes for optimal cell recovery and viability. To ensure best results when using Gibco cryopreserved human hepatocytes, use a one step centrifugation pro

Gibco Cell Therapy Systems (CTS) ACK (Ammonium-Chloride-Potassium) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. The complete formulation an

CTS B-27 Supplement XenoFree is a cGMP-manufactured, serum-free supplement designed to meet regulatory requirements for cell therapy manufacturing. This supplement supports the growth, proliferation, and differentiation of various cell types derived from

CTS B-27 Supplement XenoFree, minus insulin, is a cGMP-manufactured, serum-free supplement designed to meet regulatory requirements for cell therapy manufacturing. It supports the growth and differentiation of a variety of cell types derived from pluripo

Gibco Cell Therapy Systems (CTS) Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents

Gibco Cell Therapy Systems (CTS) L‐Glutamine is a ready-to-use 200-mM stock solution that acts as a cell culture supplement. L-glutamine is an amino acid that is required for cell culture. It is involved in the formation of purine and pyrimidine nucleoti

Gibco CultureCEPT Supplement reduces cellular stress and improves viability of stem cells, differentiated cells, and primary cells during a wide variety of handling and processing steps where cell damage and death can limit the success of a cell culture

This distilled water is high-quality water for use as a solvent in the preparation of cell culture media and laboratory reagents. Available in many sizes to meet your needs, this distilled water features:Cell culture qualityProven performanceSuitable for

Dopaminergic Neuron Maturation Supplement is a component of the Gibco™ PSC Dopaminergic Neuron Differentiation Kit that is also offered separately. It enables maturation of excess floor plate progenitor cells to midbrain dopaminergic neurons.

Gibco EfficientFeed A+ 3X Supplement is a single-part liquid supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization. Compared to original CHO CD EfficientFeed

Gibco EfficientFeed B+ 3X Supplement is a single-part liquid supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization. Compared to original CHO CD EfficientFeed

Gibco EfficientFeed C+ 2X Supplement is a single-part liquid supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization. This product is designed to work with CHO