Protein Mass Spectrometry Sample Prep

Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) provide high sensitivity and mass accuracy; however, interfering molecules from the sample can result in reduced sensitivity and poor-quality spe

Analysis of human fluids is often complicated by the presence of high concentrations of serum proteins, including albumin and IgG that can make up more than 70% of total serum protein. Removal of albumin, immunoglobulins, and other abundant proteins is e

Use the Thermo Scientific™ Pierce™ HeLa Protein Digest Standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples. The Thermo Scientific Pierce HeLa Protein Digest S

The Pierce Quantitative Fluorescent Peptide Assay reagents include peptide assay buffer, fluorescent peptide labeling reagent, and a peptide digest assay standard for the quantitative measurement of peptide concentrations. In this assay, peptides are spe

Kit contains pre-formulated buffers, MS-grade trypsin/Lys-C enzyme mix, and peptide clean up cartridges for the processing of samples from protein lysis and digestion through peptide clean-upThe single-use components are conveniently provided in labeled

Kit provides control reagents for installation and QC of the AccelerOme Automated Sample Prep SystemThe AccelerOme System Test Kit is required for installation of the AccelerOme Automated Sample Prep SystemReady-to-use solutions provided in a tray that i

The AccelerOme TMT11plex MS Sample Preparation Kit integrates pre-formulated buffers, MS-grade trypsin/Lys-C enzyme mix, and peptide clean-up cartridges with TMT11plex label reagents for reproducible sample preparation of TMT-labeled peptides. Features o

The AccelerOme TMTpro 16plex MS Sample Preparation Kits integrate pre-formulated buffers, MS-grade trypsin/Lys-C enzyme mix, and peptide clean-up cartridges with TMTpro 16plex label reagents for reproducible sample preparation of TMT-labeled peptides. Th

Thermo Scientific™ MALDI Matrices are highly purified, recrystallized reagents packaged in convenient single-use tubes sufficient for analyzing up to 96 samples by matrix assisted laser desorption ionization. The MALDI method for MS peptide analysis is n

Thermo Scientific™ MALDI Matrices are highly purified, recrystallized reagents packaged in convenient single-use tubes sufficient for analyzing up to 96 samples by matrix assisted laser desorption ionization. The MALDI method for MS peptide analysis is n

Proteomic sample preparation for MS analysis is complex, resulting in challenges with reproducibility and peptide quality. For MS sample preparation methods that utilize magnetic beads, a major challenge is the compatibility of the beads with the harsh c

EasyPep Mini MS Sample Prep Kits enable efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized prot

Thermo Scientific™ Formic Acid is high-purity solvent supplied in glass ampules as a convenient, contamination-free alternative for preparing elution solvents for HPLC separations of protein and peptides. Formic acid is a component found in reverse-phase

Mass spectrometry (MS) is a key tool for identifying sites of protein phosphorylation and quantifying phosphorylation changes. However, MS analysis of protein phosphorylation is challenging due to the low stoichiometry, high hydrophilicity, poor ionizati

The Invitrosol™ LC/MS (Liquid Chromatorgraphy/Mass spectrometry) Protein Solubilizer Kit contains a novel, proprietary surfactant blend for simplified preparation of hydrophobic and hydrophilic proteins or peptides for ESI (Electrospray Ionization) mass

Thermo Scientific™ MALDI Matrices are highly purified, recrystallized reagents packaged in convenient single-use tubes sufficient for analyzing up to 96 samples by matrix assisted laser desorption ionization. The MALDI method for MS peptide analysis is n

Protein characterization, identification, and quantification by MS begins with efficient, reproducible protein digestion. Sequence specific proteases are utilized to cleave proteins into smaller fragments or peptides. Trypsin is the protease of choice fo

The Thermo Scientific Pierce 6 Protein Digest Standard, Equimolar, LC-MS Grade, is a highly validated equimolar protein digest mixture for use as a quality control sample for mass spectrometry (MS) analysis of proteomic samples.Positive control sample -

Concentrations of albumin and IgG can make up more than 70% of total serum proteins and often mask lower quantity proteins during protein-profiling experiments. Optimize your serum sample prep by removing albumin or IgG quickly and conveniently. By using

Thermo Scientific™ Pierce APTS (3-aminopropyltriethoxysilane) is a silylation reagent for coating glass and silica surfaces to add primary amines, which can be used to crosslink and immobilize proteins and other molecules. APTS (3-aminopropyltriethoxysil

The Thermo Scientific Pierce Aquaporin Z Membrane Protein Standard is a highly validated pure membrane protein formulated for direct infusion native mass spectrometry experiments.Features of Pierce Aquaporin Z Membrane Protein Standard include:Positive c

The Thermo Scientific Pierce BSA Protein Digest Standard, LC-MS Grade, is a validated protein digest optimized for use as a quality control sample for liquid chromatography (LC) and mass spectrometry (MS) analysis of proteomic samples.Positive control sa

Thermo Scientific™ Pierce Chromatography Cartridges are convenient, reliable, ready-to-use, pre-packed 1mL and 5mL columns of sample-prep and affinity-purification resins for manual or automated liquid chromatography. The cartridge-fittings are compatibl

Thermo Scientific Pierce Dimethylformamide (DMF) is a sequencing-grade preparation with high purity and consistent absorptivity properties for use in HPLC and mass spectrometry methods. This sequencing-grade dimethylformamide is specially purified by a p

Thermo Scientific™ Pierce Dimethylsulfoxide (DMSO) is a sequencing-grade preparation with high purity and consistent absorptivity properties, for use in HPLC and mass spectrometry methods. A sequencing-grade preparation with high purity for use in tradit

Thermo Scientific™ Pierce Dimethylsulfoxide (DMSO) is a sequencing-grade preparation with high purity and consistent absorptivity properties for use in HPLC and mass spectrometry methods. A sequencing-grade preparation with high purity for use in traditi

Improved mass accuracy - extended mass range improves mass accuracyPositive and negative mode calibration - single formulation with extended mass ranges for both ionization calibration modesHighly validated - high-quality, rigorously-tested formulation w

Extended mass range improves mass accuracyPositive and negative mode calibration, a single formulation with extended mass ranges for both ionization calibration modesHigh-quality, rigorously-tested formulation with lot-specific certificates of analysisBo

The Thermo Scientific Pierce HeLa Digest/PRTC Standard is a validated complex mammalian protein digest containing additional PRTC heavy isotope-labeled peptides designed for LC/MS performance testing.Features of HeLa Digest/PRTC Standard include:Convenie

In order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-

Thermo Scientific™ Pierce LTQ ESI Positive Ion Calibration Solution is a mixture of highly purified ionizable molecules specifically designed for positive mode calibration of Thermo Scientific™ ion trap and orbitrap instruments. The Pierce LTQ ESI Positi

Thermo Scientific™ Pierce LTQ Velos ESI Positive Ion Calibration Solution is a mixture of highly purified ionizable molecules designed for positive mode calibration of Thermo Scientific™ LTQ Velos series mass spectrometer instruments. The Pierce LTQ Velo

Get maximum activity and stability in proteomic applications with Thermo Scientific™ Pierce™ LysN Protease, MS Grade, a highly purified native Lys-N protease. Thermo Scientific Pierce LysN Protease, MS Grade, is highly purified native Lys-N protease that

Immunoprecipitation is often necessary for the detection and quantitation of low-abundance proteins or rare signaling events. Mass spectrometry analysis relies on two methods for the detection of low-abundance proteins: single reaction monitoring (SRM) a

Thermo Scientific™ Pierce ESI Negative Ion Calibration Solution is a mixture of highly purified ionizable molecules designed for negative mode calibration of Thermo Scientific™ LTQ series mass spectrometer instruments. The Pierce ESI Negative Ion Calibra

Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. The polymer-based hydrophobic resin contained in each column provides excel

Thermo Scientific™ Pierce Peptide Retention Time Calibration Mixture enables reversed phase liquid chromatography (LC) and mass spectrometry (LC-MS) users to optimize and confirm correct operation of their equipment. The Pierce Peptide Retention Time Cal

Thermo Scientific Pierce Protein Precipitation Plates provide an easy, 96-well format for simple, rapid and automatable protein precipitation and filtration (purification) from plasma, serum and other biological fluid samples. Pierce™ Protein Precipitati

Thermo Scientific Pierce Pyridine (PY) is a sequencing-grade preparation with high purity and consistent absorptivity properties for use in HPLC and mass spectrometry methods. Highlights:Water-miscible organic solvent for a variety of molecular and prote

Pierce colorimetric or fluorescent quantitative peptide assays are easy mix-and-read microplate assays with stable signals for accurate and robust measurement of peptide digest samples. A high-quality peptide digest reference standard is provided as a st

Thermo Scientific™ Pierce Reserpine Standard for LC-MS is an exact solution of reserpine specifically designed for performance evaluation of mass spectrometers, including the Thermo Scientific ion trap and TSQ series of instruments.Additional Information

A pre-formulated mixture of nine small-molecule standards to assess system performance in both positive and negative ionization modes for TSQ Triple Quadrupole and Orbitrap Exploris mass spectrometersComprehensive mixture of nine small-molecule compounds

Thermo Scientific™ Pierce Trifluoroacetic Acid (TFA) is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations. For complex peptide separations, the key

Thermo Scientific™ Pierce Trifluoroacetic Acid (TFA) is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations. For complex peptide separations, the key

Thermo Scientific™ Pierce Triple Quadrupole Calibration Solution is a mixture of high purity, ionizable components specifically designed for positive mode calibration of Thermo Scientific™ Triple Stage Quadrupole instruments. The Pierce Triple Quadrupole

The Thermo Scientific Pierce Triple Quadrupole Calibration Solution, Extended Mass Range, is a mixture of 14 highly pure, ionizable components (mass range: 69 m/z to 2800m/z) designed for both positive and negative ionization calibration of Thermo Scient

The Thermo Scientific Pierce Yeast Digest Standard is a lyophilized yeast peptide mixture ideal for monitoring LC/MS system performance.Pierce Yeast Digest Standard features include:Positive control sample—well-characterized eukaryotic proteome sample pr

Thermo Scientific™ MALDI Matrices are highly purified, recrystallized reagents packaged in convenient single-use tubes sufficient for analyzing up to 96 samples by matrix assisted laser desorption ionization. The MALDI method for MS peptide analysis is n

Thermo Scientific SureQuant IP-MS Sample Preparation kits (Protein A/G or streptavidin) provide an optimized solution for the immunoprecipitation and MS peptide preparation of samples upstream of LC-MS/MS analysis. These kits have been rigorously verifie