Nucleic Acid Labeling and Detection

Achieve accurate and precise quantification of dsDNA with Qubit dsDNA BR (Broad Range) Assay Kits. These dsDNA quantification kits enable quick and selective detection of low and high abundance DNA samples, and can distinguish dsDNA from ssDNA, RNA, prot

Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and the Quant-iT Broad-Range dsDNA Assay Kit provide concentrated assay reagent, dilution buffer, and pre-diluted DNA stand

Experience easy, accurate, and precise quantification of dsDNA with Qubit 1X dsDNA HS (High Sensitivity) and Qubit 1X dsDNA BR (Broad Range) Assay Kits. The 1X formulation provides a ready-to-use working solution that enables simple dsDNA sample quantifi

Achieve accurate and precise quantification of RNA with Qubit RNA HS, BR, and XR Assay Kits. These RNA quantification kits enable quick and selective detection of low and high abundance RNA samples, and can distinguish RNA from DNA, protein, and free nuc

Quantify as little as 1 ng of RNA with the Quant-iT RiboGreen RNA Assay Kit or the RediPlate 96 RiboGreen RNA Quantitation Kit. These RNA quantification kits contain Quant-iT RiboGreen RNA reagent, which is one of the most sensitive detection dyes for th

Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers. Rapidly detect as lit

RNase-free TE Buffer (20X) is an essential component of Qubit and Quant-iT assays, including PicoGreen, RiboGreen, and Qubit dsDNA HS. It is offered here as a standalone solution to support quantification of dsDNA, ssDNA and RNA. Note: For dilution instr

Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA). The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminesce

Perform easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins with Thermo Scientific™ Pierce RNA 3' End Desthiobiotinylation Kit.Additional Information and Online Ordering Th

The Alexa Fluor 488 Oligonucleotide Amine Labeling Kit provides a simple method for labeling amine-modified oligonucleotides with our bright, photostable, green-fluorescent Alexa Fluor 488 dye (which is spectrally similar to fluorescein). Oligonucleotide

The ARES™ Alexa Fluor™ DNA Labeling Kit provides a versatile, two-step method for labeling DNA with our Alexa Fluor™ dyes. In the first step, an amine-modified nucleotide is incorporated into DNA using conventional enzymatic labeling methods. In the seco

The ARES™ Alexa Fluor™ DNA Labeling Kit provides a versatile, two-step method for labeling DNA with our Alexa Fluor™ dyes. In the first step, an amine-modified nucleotide is incorporated into DNA using conventional enzymatic labeling methods. In the seco

The BioNick™ DNA Labeling System is ideal for generating biotinylated DNA probes by nick translation optimized for use in non-radioactive in situ hybridizations. These probes can also be used in Southern or northern blots, plaque lifts, colony hybridizat

Random primers (octamers) are annealed to the denatured DNA template and extended by Klenow fragment in the presence of biotin-14-dCTP to produce sensitive biotinylated-DNA probes for use in the nonradioactive detection of DNA and RNA. With the BioPrime™

Previous protocols have required large amounts of very high quality DNA, which is often difficult to obtain from FFPE material. With a random prime amplification (RPA) method, using labeling mixes specifically formulated for FFPE samples, BioPrime™ Total

The BioPrime Total Genomic Labeling Module is part of the system that offers users the following advantages:Higher signal to noise with Alexa Fluor™ 3 and 5 dyesLess channel bias due to novel reaction formulationWidest range of input material (50ng - 3μg

These complete, all-inclusive kits are optimized to work across the widest range of sample input material with no need for pre-amplification. Highly concentrated exo-Klenow fragment of DNA polymerase I in a component limited reaction allow for consistent

Thermo Scientific™ Fermentas Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method originally developed by Feinberg and Vogelstein. The primary

Thermo Scientific™ Pierce Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acid probes in various membrane blotting applications. The Thermo Scientific Chemiluminescent Nucleic Acid Detection Mod

The Click-iT Nascent RNA Capture Kit, uses proprietary Click-iT chemistry to capture with high efficiency and sensitivity newly minted RNAs. By the Click-iT approach studies on high resolution analysis of gene regulation can be performed along with RNA r

The Click-iT RNA Alexa Fluor 488 HCS Assay enables detection of global RNA transcription temporally and spatially in cells (PNAS (2008) 105:15779-84). The ability to detect newly synthesized RNA or changes in RNA levels resulting from disease, environmen

The Click-iT RNA Alexa Fluor 488 Imaging Kit enables detection of global RNA transcription temporally and spatially in cells and tissues (PNAS (2008) 105:15779-84). The ability to detect newly synthesized RNA or changes in RNA levels resulting from disea

The Click-iT RNA Alexa Fluor 594 Imaging Kit enables detection of global RNA transcription temporally and spatially in cells and tissues (PNAS (2008) 105:15779-84). The ability to detect newly synthesized RNA or changes in RNA levels resulting from disea

Choose from three, single-color FISH Tag DNA kits with Alexa Fluor dye options (green, orange, or red). The FISH DNA multicolor kit contains four spectrally distinct dyes (green, orange, red, and deep-red) to allow simultaneous localization of multiple s

Choose from two, single-color FISH Tag RNA kits with Alexa Fluor dye options (green or red). The FISH RNA multicolor kit contains four spectrally distinct dyes (green, orange, red, and deep-red) to allow simultaneous localization of multiple sequence-spe

Quantitation of cellular DNA is rapid, and all manipulations can be carried out in microplate wellsCells are lysed by freezing them in distilled water, diluted dye solution is then added to lysed cells, and fluorescence is measuredBiochemical Toxicology

Thermo Scientific™ LightShift Chemiluminescent EMSA Kit is an extraordinarily robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions. The kit includes reagents

Thermo Scientific™ LightShift Chemiluminescent EMSA Kit is an extraordinarily robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions. The kit includes reagents

The Nick Translation System is ideal for both radioactive and non-radioactive labeling of DNA. The Nick Translation System:Provides five pre-mixed nucleotide solutions for flexibility in labeled nucleotide incorporationYields >108 cpm/μg control DNA usin

Thermo Scientific™ North2South Random Prime DNA Biotinylation Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridization and detection methods. Random heptanucleotides containing a

Thermo Scientific™ North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results. The Thermo Scient

Nucleic Acid Detection Blocking Buffer is intended for use with the Thermo Scientific Chemiluminescent Nucleic Acid Detection Module Kit, of which it is also a component. Non-specific binding of probe to membrane can be minimized by incubating the membra

Thermo Scientific™ Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods. TdT exhibits a substrate pre

Thermo Scientific™ Pierce RNA 3' End Biotinylation Kit is optimized for labeling the 3-prime terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions. The complete kit contain

Make DNA quantitation easy and accurate in a ready-to-go 1X formulation with the Quant-iT 1X dsDNA HS (High-Sensitivity) and Quant-iT 1X dsDNA BR (Broad Range) Assay kits. Both kits provide a ready-to-use working solution. Simply load your sample, add th

The Quant-iT™ microRNA Assay Kit allows easy and accurate quantification of microRNA (miRNA), even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for microRNA over rRNA or large mRNA (>1000 bp). Although the reagent is not exclusively selective for miRNA, we have been able to reproducibly quantify miRNA in pure samples at levels as low as 0.5 ng following the supplied protocol, even in the presence of mRNA.Features of Quant-iT™ microRNA Assay Kit: Accurately detects as little as 0.5 ng miRNA in a microplate wellCore dynamic range of 5–500 ng/mLAccurate results for sample concentrations from 50 ng/mL to 100μg/mL The Quant-iT™ microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent 1:200, load 200μL into the wells of a microplate, add 1–20μL of sample, mix, then read the fluorescence. The assay is performed at room temperatu

Quantify ssDNA with ease using the Quant-iT OliGreen ssDNA Assay kit and reagent. This assay kit and reagent enable sensitive quantitation of single-stranded DNA without the need for a highly concentrated sample. As little as 100 pg/mL can be quantitated

The Quant-iT PicoGreen ReadyPlate is a preloaded 96-well plate that offers a rapid, ultra-sensitive, and selective method for double-stranded DNA quantification. The plate is prefilled with buffer and PicoGreen reagent, which exhibits bright green fluore

The Quant-iT RiboGreen ReadyPlate is a preloaded 96-well plate that offers a rapid, simplified, and highly sensitive method for RNA quantification. The ReadyPlate is prefilled with buffer and RiboGreen reagent, which exhibits bright green fluorescence up

Enable easy and accurate RNA quantitation with the Quant-iT RNA Assay Kit, which is also available in broad range (BR) and extended range (XR) configurations, as well as a stand-alone high sensitivity (HS) reagent. These Quant-iT RNA quantification kits

Achieve precise quantitation of dsDNA or RNA samples with stand-alone Qubit nucleic acid standards. The Qubit standards can be used in conjunction with different Qubit or Quant-iT nucleic acid quantitation assay kits and offer stand-alone versions of the

Quickly and easily determine RNA degradation with the Qubit RNA IQ Assay Kit and Qubit RNA IQ Standards. RNA integrity and quality (IQ) information is assessed using the assay kit's two unique dyes: one dye that binds to large and/or highly structured RN

The Qubit ssDNA Assay Kit is designed specifically for use with the Qubit Fluorometer, but may be used with any fluorometer or fluorescence plate reader. The Qubit™ ssDNA Kit is ideal for quantitating single-stranded DNA or oligonucleotides. However, it

ReadyProbes In Situ Hybridization (ISH) Blocking Solution minimizes non-specific background staining in chromogenic in situ hybridization (CISH) or fluorescence in situ hybridization (FISH) proceduresIt is provided as a 5X solution that is diluted to 1X

For the labeling of siRNA with Cy™3. Includes sufficient reagents for labeling 65μg of siRNA. Invitrogen™ Labeled siRNA can be used to analyze siRNA subcellular localization, stability, and transfection efficiency. In addition, fluorescently labeled siRN

The kit includes Invitrogen's proprietary RNase H reduced activity SuperScript™ II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix– protocol. Designed to consistently produce higher yiel

Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA). The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminesce

ULYSIS nucleic acid labeling kits provide a unique method for attaching a fluorescent dye to nucleic acids. The labeling reagent in the kit reacts with the N7 of guanine to form a stable coordination complex, and the reaction is simple and fast - just he