Gel Electrophoresis Reagents and Buffers

Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.Pre-mixed buffers are a convenient way to ensure high-quality, consistent electrophoresis resultsMade with high

NuPAGE MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Novex Bis-Tris gels only. NuPAGE MES SDS Running Buffer is recommended for separating small- to medium-sized proteins.Use of MES buffer allows proteins to run faster Ensures

NuPAGE Sample Reducing Agent contains 500mM dithiothreitol (DTT) at a ready-to-use, 10X concentration in a stabilized liquid form. Use NuPAGE Sample Reducing Agent when running proteins on NuPAGE Novex Bis-Tris gels to maintain protein samples in a reduc

Thermo Scientific™ Pierce LDS Sample Loading Buffer (4X) is a nonreducing lithium dodecyl sulfate sample loading buffer optimized for use with Pierce Protein SDS-PAGE Gels. LDS Sample Buffer, Non-Reducing (4X) is a convenient sample buffer for use in SDS

Novex IEF Anode Buffer (50X) is optimized pre-mixed IEF anode buffer for using with Novex IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.Isoel

1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

Bolt™ Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditionsSimilar to NuPAGE™ Bis-Tris gels, Bolt™ Bis-Tris Plus gels are designed to deliver consistent gel perfo

Comprehensive - contains markers with a wide range of high molecular weights, providing nine protein bands in the range of 30 to 460kDaConvenient - a ready-to-use formulation eliminates the need to heat, reduce, or add sample buffer prior to useEfficient

PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. Each 10X PBS solution is

Thermo Scientific™ Pierce Silver Stain Kit is a rapid, ultra-sensitive and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results with many 1D and 2D gel types for both first-time and experien

Invitrogen Power Blotter 1-Step Transfer Buffer is designed for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Power Blotter System. Transfer is compatible with commonly used detectio

Leak-free, confident handcasting of your polyacrylamide gels with the Invitrogen™ SureCast™ Handcast System. SureCast Sealing Pads are a replacement set of foam gaskets for use in the SureCast Handcast Station. Comes in a package of two (2) sealing pads.

About Bolt™ Bis-Tris Plus GelsBolt™ Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Similar to NuPAGE™ Bis-Tris gels, Bolt™ Bis-Tris Plus gels are des

About Bolt™ Bis-Tris Plus GelsBolt™ Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Similar to NuPAGE™ Bis-Tris gels, Bolt™ Bis-Tris Plus gels are des

Novex™ Bolt™ buffers and sample reducing agents are optimized for use with Bolt Bis-Tris Plus gels, and are available in a variety of formats. About Bolt™ Bis-Tris Plus GelsBolt™ Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal sep

When combined with 10% methanol, Bolt Transfer Buffer can be used with Bolt Mini Blot Module for wet transferCan also be used with Bolt Antioxidant to enhance transfer of reduced proteins to membranes1D Gel Electrophoresis, Protein Gel Electrophoresis, P

GelCode Blue Safe Protein Stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and membranes. This protein-specific stain allows bands to be viewed directly on a gel or mem

IEF Cathode Buffer pH 3-10 (10X) is optimized pre-mixed IEF cathode buffer for using with Novex pH 3-10 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent

Mark12 Unstained Standard is supplied ready-to-use with either NuPAGE™, tris-glycine, or tricine gels. Coomassie™, silver, or other protein staining methods are required to visualize this standard.12 polypeptides resolved into sharp, tight bands in the r

The buffer is used along with 10% DDM or 5% Digitonin detergents to solubilize hydrophobic or membrane proteins. This buffer is also included in the NativePAGE Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE Sample Pre

Novex IEF Cathode Buffer pH 3-7 (10X) is optimized pre-mixed IEF cathod buffer for using with Novex pH 3-7 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consist

Novex IEF Sample Buffer pH 3-7 (2X) is optimized pre-mixed IEF sample buffer for using with Novex pH 3-7 IEF gels. IEF is a sensitive technique which is affected by many factors.Isoelectric Focusing, Protein Gel Electrophoresis, Proteins, Expression, Iso

Novex Tricine SDS Running Buffer (10X) is formulated for separation of proteins in their denatured state on tricine gels. Tricine gels are specifically designed for the resolution of low molecular weight proteins. In this buffer system, tricine substitut

In the tricine system developed by Schaegger and von Jagow (1), tricine replaces glycine in the running buffer. This results in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.1D Gel Elect

1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

Add to running buffer in the upper (cathode) buffer chamber when performing protein gel electrophoresis under reducing conditions.Use with samples reduced using NuPAGE Sample Reducing Agent or other reducing agentsOptimized for neutral pH of NuPAGE Novex

NuPAGE MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Novex Bis-Tris gels only. NuPAGE™ MOPS SDS Running Buffer is recommended for separating small- to medium-sized proteins.Get consistent, convenient, high-quality resultsPre-

NuPAGE Transfer Buffer can be used with the Novex Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE Antioxidant may be used with NuPAGE Transfer Buffer to enhance transfer of reduced proteins to memb

NuPAGE Novex Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer Premixed buffers are a convenient way to ensure high-quality, consistent electrophoresis results1D Gel El

Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a

Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color (blue, orange, green, pink) protein standard containing 10 prestained proteins (10 to 260 kDa) for use in gel electrophoresis and western blotting. The protein ladder is supplie

Thermo Scientific™ Spectra Multicolor Low Range Protein Ladder is a mixture of six (6) proteins and synthetic peptides (1.7 to 40kDa) for use as size standards for peptides and small proteins in gel electrophoresis and Western blotting. This prestained p

Thermo Scientific™ Pierce Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis. Ammonium persulfate (APS) is an oxidizing

The standard proteins can be resolved into sharp bands in SDS-PAGE and visualized using a UV transilluminator or commonly used laser scanner systems. Seven tight, clear bands that resolve well in NuPAGE™ Bis-Tris Gels and Novex Tris-Glycine GelsVisu

The BenchMark His-tagged Protein Standard can be used with NuPAGE™ Gels and Tris-Glycine Gels.Verification of his-tagged fusion protein detection using InVision™ In-Gel Stain or an Anti-His(C-term) antibody Ten sharp and clear bands in the range of 10-16

The BenchMark Pre-Stained Protein Ladder consists of 10 proteins ranging in apparent molecular weight from 6 to 180 kDa designed for use with Tris-Glycine gels.Proteins covalently bound to a blue dye for easy visualizationOne protein band coupled to a pi

The BenchMark Protein Ladder consists of 15 recombinant proteins ranging in molecular weight from 10 to 220kDa and can be used with NuPAGE™,Tris-Glycine, or Tricine gels.Extra-intense 20- and 50-kDa orientation bands to ensure proper identification of ea

Our dry-blend buffer packs are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water, and stir to dissolve. No need to make and store large volumes of stock solution in advance of daily needs. Benefits of ready-t

Our dry-blend buffer packs are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water, and stir to dissolve. No need to make and store large volumes of stock solution in advance of daily needs. Benefits of ready-t

Our dry-blend buffer packs are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water, and stir to dissolve. No need to make and store large volumes of stock solution in advance of daily needs. Benefits of ready-t

Our dry-blend buffer packs are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water, and stir to dissolve. No need to make and store large volumes of stock solution in advance of daily needs. Benefits of ready-t

Our dry-blend buffer packs are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water, and stir to dissolve. No need to make and store large volumes of stock solution in advance of daily needs. Benefits of ready-t

Our dry-blend buffer packs are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water, and stir to dissolve. No need to make and store large volumes of stock solution in advance of daily needs. Benefits of ready-t

Our dry-blend buffer packs are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water, and stir to dissolve. No need to make and store large volumes of stock solution in advance of daily needs. Benefits of ready-t

Our dry-blend buffer packs are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water, and stir to dissolve. No need to make and store large volumes of stock solution in advance of daily needs. Benefits of ready-t

System: SDS-PAGESize Range: 14 to 180 kDaStain Type: UnstainedReady to Load: NoGel Compatibility: NuPAGE™ Tris-Acetate Gels, NuPAGE™ Bis-Tris Gels, Novex™Tris-Glycine Gels, SDS-PAGE Gels, Novex™Tricine Gels1D Gel Electrophoresis, Protein Gel Electrophore

Citrate buffer is used in Heat Induced Epitope Retrieval (HIER) methods. HIER is used to reverse the loss of antigenicity that occurs with some epitopes in formalin-fixed paraffin embedded tissues. During epitope retrieval, tissue slides are immersed in

The Colloidal Blue Staining Kit allows detection of nanogram levels of protein with minimal effort and requires only water to destain. The Colloidal Blue stain uses colloidal chemistry that reduces free dye in solution and improves the protein-to-dye bin

Molecular Probes proprietary Coomassie Fluor Orange protein gel stain provides fast, simple, sensitive staining of proteins separated by SDS-PAGE. After electrophoresis, the gel is stained, rinsed and photographed. No separate fixation or destaining step

Ready-to-use detergent solution improves solubility of hydrophobic and membrane proteins during sample preparationThe samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE Sample Prep Kit are compatible with NativePAGE Novex Bis-Tris Gels for na