Crosslinking, Labeling and Protein Modification

Thermo Scientific™ Pierce AMAS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a very short spacer arm (4.4 Ås). Highlights:Reactive groups: NHS ester and maleimideReactive toward: amino and

Alexa Fluor antibody labeling kits are designed for efficiency and reliability, delivering ready-to-use labeled proteins in just 30 minutes, with minimal hands-on time. The kits have been redeveloped with user-friendly, advanced purification columns that

Thermo Scientific™ Pierce Sulfo-NHS is pure N-hydroxysulfosuccinimide, the essential compound needed to prepare water-soluble, amine-reactive NHS-ester crosslinkers or to enhance conjugation efficiencies when using the carbodiimide EDC. Highlights:Forms

Invitrogen Dylight Antibody Labeling kits provide everything needed to label 1 mg of antibody or protein with amine-reactive Dylight dye and subsequent free dye removal. Choose from a variety of Dylight fluorophores across the spectrum to covalently labe

Thermo Scientific™ Pierce SATA is a short-chain (2.8 Å spacer arm) reagent for covalent modification of primary amines and addition of a stable yet exposable sulfhydryl group to facilitate heterobifunctional crosslinking strategies. Highlights:Reacts wit

Thermo Scientific™ Pierce Sulfo-SANPAH is a long-arm (18.2 Å) crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide. Highlights:Optimal photolysis occurs at 320-350nm, which limits damage to

Proteinase K digests proteins such as ribonucleases that could interfere with the quantitation of RNA using QuantiGene assays.

TMT10plex Isobaric Label reagents and kits contain ten isobaric compounds with the same mass and chemical structure (i.e., isotopomeric) composed of an amine-reactive NHS-ester group, a spacer arm, and a mass reporter. Up to ten different peptide samples

Pre-formulated TEAB and 50% hydroxylamine designed for use in TMT labeling and quenching of peptides. Triethylamonium bicarbonate (TEAB) is a 10X dissolution buffer for isobaric mass tag labeling experiments. 50% hydroxylamine is a 10X quenching reagent

The amine-reactive 1-pyrenebutanoic acid, succinimidyl ester can be used to create environment-sensitive bioconjugates with this unique fluorophore.

Oregon Green™ 488 can be used as a reference standard for Oregon Green™ 488 conjugates or a pH sensor of moderately acidic solutions (pKa ∼4.7)

The amine-reactive version of digoxigenin (3-amino-3-deoxygigoxigenin hemisuccinamide, SE) can be used to attach this important hapten to biomolecules of interest for subsequent detection with an anti-digoxigenin antibody.

Our 4-acetamido-4'-maleimidylstilbene-2,2'- disulfonic acid is a thiol-reactive reagent that is water soluble, with high polarity and membrane impermeability. This polysulfonated dye is useful for determining whether thiol-containing proteins and polypep

Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.

Fluorescein is a common green-fluorescent derivatization reagent, while succinimidyl esters are frequently used for the labeling of the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. This SFX su

5(6)-TAMRA contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. This bright, orange-fluorescent dye produces conjugates with absorption/emission maxima of ∼555/580 nm.

The amine-reactive 6-(tetramethylrhodamine-5-(and-6)-carboxamido)hexanoic acid, succinimidyl ester (5(6)-TAMRA-X, SE) can be used to can be used to create bright red-orange fluorescent bioconjugates with excitation/emission maxima ∼555/580 nm.

The amine-reactive form of 5-CR 6G is used for oligonucleotide labeling and automated DNA sequencing applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine™ rhodamine B conjugates, but somewhat shorter-wavelengt

Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.

The carboxylic acid of 5-ROX is used for oligonucleotide labeling and automated DNA sequencing applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine™ rhodamine B conjugates, but somewhat shorter-wavelength spec

The isomeric 5-ROX, SE is an amine-reactive form of carboxy-X-rhodamine and is widely used for oligonucleotide labeling and automated DNA sequencing applications.

Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.

Tetramethylrhodamine (TMR, TRITC) has been a widely used fluorophore for preparing bioconjugates, especially fluorescent antibody and avidin derivatives used in immunochemistry. Under the name TAMRA, the carboxylic acid of 5-TAMRA has also achieved promi

The amine-reactive 5-TAMRA, SE and its conjugates yield bright, pH-insensitive orange-red fluorescence (approximate excitation/emission maxima ∼546/579) with good photostability.

The single isomer, 6-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In additio

This single isomer preparation of the amine-reactive dye 6-JOE, SE has fluorescence characteristics that are red-shifted as compared to fluorescein (excitation/emission is 522/550 nm) and is one of the traditional fluorophores used in automated DNA seque

The excitation and emission spectra of carboxyrhodamine 6G (CR 6G) fall between those of fluorescein and tetramethylrhodamine. With a peak absorption at 525 nm, conjugates of carboxyrhodamine 6G are an excellent match to the 514 nm spectral line of the a

The isomeric 6-ROX, SE is an amine-reactive form of carboxy-X-rhodamine and is widely used for oligonucleotide labeling and automated DNA sequencingapplications.

The isomeric 6-TAMRA, SE is an amine-reactive form of tetramethylrhodamine. The single-isomer preparations of TAMRA are most important for high-resolution techniques such as DNA sequencing and capillary electrophoresis. 6-TAMRA is one of the traditional

7-Amino-4-methylcoumarin (AMC) can be used as a fluorescence reference standard for AMC-based enzyme substrates including AMC-based caspase substrates. The fluorescence spectra of AMC is not subject to variability due to pH-dependent protonation/deproton

Thermo Scientific™ Pierce 8M Guanidine-HCl Solution is an accurately formulated, ready-to-use solution of guanidine hydrochloride, that can be easily diluted and pH-adjusted to any concentration below 8M. Highlights:Any concentration below 8 M can be qui

Acryloyl-X, SE (6-((acryloyl)amino)hexanoic acid, succinimidyl ester) is an amine-reactive molecule that enables crosslinking of acrylamide to proteins and other biomolecules containing amines. The succinimidyl ester of acryloyl-X covalently reacts with

Alexa Fluor™ 350 is a blue fluorescent dye with moderate photostability and excitation that matches the 350 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 350 dye is water soluble and pH-insensitive from pH 4

Alexa Fluor™ 350 Hydrazide is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins. Alexa Fluor™ 350 is a blue fluorescent dye with excitation ideally suited to the UV laser line. Used for st

Alexa Fluor™ 350 is a blue-fluorescent dye. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 350 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Alexa Fluor™ 350

Alexa Fluor™ 405 Cadaverine is useful as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety. Alexa Fluor™ 405 is a bright, violet dye with excitation ideally suited to the 405 nm laser line. Used for stable signal gen

Alexa Fluor™ 405 is a blue-fluorescent dye optimal for use with the 405 nm violet laser. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 405 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive

Alexa Fluor 420 NHS Ester is a fluorescence-based amine-reactive dye specifically optimized for multiplex immunohistochemistry and spatial biology. With excitation/emission maxima of 420/500 nm, this dye is a good match for the CFP filter on fluorescence

In addition to reactive dye formulations, Invitrogen offers Alexa Fluor™ 430 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection.The NHS ester (or succinimidyl

The SDP (sulfodicholorphenol) ester is currently the most hydrolytically stable amine-reactive moiety available from Invitrogen. Conjugates produced with the Alexa Fluor™ 488 5-SDP ester produce the same strong amide bond between the dye and the compound

Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond

The green-fluorescent Alexa Fluor™ 488 alkyne is reactive with azides via a copper-catalyzed click reaction. In addition to being an exceedingly bright and photostable fluorophore for use with flow cytometry, microscopy and HCS, Alexa Fluor™ 488 can also

The green-fluorescent Alexa Fluor™ 488 azide is reactive with terminal alkynes via a copper-catalyzed click reaction. In addition to being an exceedingly bright and photostable fluorophore for use with flow cytometry, microscopy and HCS, Alexa Fluor™ 488

Alexa Fluor™ 488 C5 Maleimide is a bright, green fluorescent dye with excitation ideally suited to the 488 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 488 dye is water soluble and pH-insensitive from pH 4

Alexa Fluor™ 488 Cadaverine is useful as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety. Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. Used for stable

Alexa Fluor™ 488 Hydrazide is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins. Alexa Fluor™ 488 is a bright, green fluorescent dye with excitation ideally suited to the 488 nn laser line

Alexa Fluor™ 488 Hydroxylamine is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins. Alexa Fluor™ 488 is a bright, green fluorescent dye with excitation ideally suited to the 488 nn laser

Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20–100 μg). The kits are available in four Alexa Fluor™ colors (or biotin) and supply everything needed for three labe