Substrates

Vivid™ Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs. Cytochrome P450 metabolism at either of two potential oxidation sites releases the highly fluorescent product. They have superior fluorescence, solubility

The Adapta™ Universal Kinase Assay Kit is a homogenous, fluorescence-based immunoassay for the detection of ADP produced by kinases or other ATP dependent enzymes.For more information, please visit www.invitrogen.com/adapta.

A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.For use in combination with the LanthaScreen™ Eu-PY20 Antibody (Cat. No. PV5696) to evaluate inhibitors

Perform a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity using Thermo Scientific™ Mammalian β-Galactosidase Assay Kit. The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-

CCF2-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF2 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF2-AM into its negatively charged form, CCF2 or

CCF2-FA (Free Acid) is a fluorescence resonance energy transfer (FRET) substrate that consists of a cephalosporin core linking B7-hydroxycoumarin to fluorescein. This product is intended for use as a beta-lactamase substrate in a GeneBLAzer™ cell-based assay. GeneBLAzer™ cell-based assays typically utilize the membrane-permeant ester forms (CCF2-AM and CCF4-AM) of the negatively charged fluorescent beta-lactamase substrates, CCF2 and CCF4. These lipophilic esters readily enter the cell, where cleavage by endogeneous cytoplasmic esterases rapidly converts them into their negatively charged forms, thereby trapping them in the cytosol. CCF2-FA is essentially the CCF2 substrate without the esters found in the AM version. CCF2-FA is de-esterified and used in cell lysate applications, bypassing loading across the cell membrane and de-esterification steps. Cell lysates are the preferred method for applications using cells that contain a cell wall. CCF2-FA can also be used as a control to acqu

CCF4-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF4 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF4-AM into its negatively charged form, CCF4, w

A peptide substrate based upon the C-terminal tail of RNA Polymerase II that is a reported substrate for Cyclin Dependent Kinases (CDK7, CDK8 and CDK9). For use with the Adapta™ Universal Kinase Assay (Cat. No. PV5099).

This CDP-Star™ chemiluminescent substrate for alkaline phosphatase let you detect alkaline phosphatase and alkaline phosphatase-labeled molecules with unparalleled sensitivity, speed, and ease.Chemiluminescent substrate provides highly sensitive replacem

CELLstart™ is a defined substrate, containing only components of human origin (xeno-free).Defined, xenofree substrate for stem cell culturecGMP Manufactured to help ensure manufacturability and traceabilityCELLstart™ is a defined substrate, containing on

A peptide substrate based upon CDC25C that is phosphorylated by CHEK1 and other kinases.For use with the Adapta™ Universal Kinase Assay (Cat. No. PV5099).

The CMAC peptidase substrate, t-BOC-Leu-Met can be used to measure peptidase activity in solution or in live cells. The substrate has an excitation/emission maxima approx. 330/403. After cleavage by peptidases, the product produces blue-fluorescence with

This CSPD™ chemiluminescent substrate for alkaline phosphatase let you detect alkaline phosphatase and alkaline phosphatase-labeled molecules with unparalleled sensitivity, speed, and ease. • Chemiluminescent substrates provide highly sensitive replaceme

The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ∼645/660). Although the substrate itself is fluorescent (excitation/emission maxima ∼460/610 nm), the differen

DiFMUP is a fluorinated 4-methylumbelliferyl phosphate (MUP) derivative that has a lower pKa than that of MUP, making DiFMUP an excellent substrate for continuously assaying acid phosphatases at low pH. The reaction product of DiFMUP has excitation/emiss

The fluorogenic DQ™ collagen can be used to directly monitor collagenase activity. DQ™ substrates are analogs of the natural substrate that have an excessive number of fluorescent dyes attached so that the fluorescence signal is almost non-existent. This

The fluorogenic DQ™ collagen can be used to directly monitor collagenase activity. DQ™ substrates are analogs of the natural substrate that have an excessive number of fluorescent dyes attached so that the fluorescence signal is almost non-existent. This

Our DQ gelatin is a fluorogenic substrate that can be used to detect protease activity in vitro with high sensitivity. This substrate consists of highly quenched, fluorescein-labeled gelatin. Upon proteolytic digestion, its bright, green fluorescence is

DQ Green BSA is a fluorogenic substrate for proteases. A strong fluorescence quenching effect is observed when proteins are heavily labeled with BODIPY dyes. Upon hydrolysis of the DQ Green BSA to single, dye-labeled peptides by proteases, this quenching

DQ ovalbumin is a fluorogenic substrate for proteases. A strong fluorescence quenching effect is observed when proteins are heavily labeled with BODIPY dyes. Upon hydrolysis of the DQ ovalbumin to single, dye-labeled peptides by proteases, this quenching

DQ Red BSA is a fluorogenic substrate for proteases. A strong fluorescence quenching effect is observed when proteins are heavily labeled with BODIPY dyes. Upon hydrolysis of the DQ Red BSA to single, dye-labeled peptides by proteases, this quenching is

The ELF 97 phosphatase substrate upon hydrolysis produces a bright and photostable yellow-green fluorescent precipitate at the site of enzymatic activity. This fluorescent precipitate has several unique spectral characteristics, including an extremely la

The EnzChek cellulase substrate was developed for simple and rapid quantitation of cellulase. The assay can be completed in 30 minutes or less. Simply mix the enzyme with the EnxChekcellulase substrate working solution, incubate, and read the results usi

The nonfluorescent EnzChek™ lipase substrate, in the presence of lipases, produces a green-fluorescent product (excitation/emission maxima ∼507/515) for accurate lipase detection in solution.Dissolve in high-quality anhydrous DMSO or chloroform to prepar

A peptide substrate based upon ezrin/radixin/moesin (ERM) that is phosphorylated by LRRK2 and LRRK2 mutants. For use with the Adapta™ Universal Kinase Assay (PV5099).

FDP is a colorless and nonfluorescent substrate for alkaline phosphatases. Sequential alkaline phosphatase mediated hydrolysis of its two phosphate substituents yields weakly fluorescent fluorescein monophosphate followed by strongly fluorescent fluoresc

Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive substrates for galactosidases. Nonfluorescent FDG is sequentially hydrolyzed by β-galactosidase, first to fluorescein monogalactoside (FMG) and then to highly fluorescent fluorescein

Fluorescein di-β-D-glucopyranoside is one of the most sensitive substrates for glucosidases. The nonfluorescent substrate is sequentially hydrolyzed by β-glucosidase, first to fluorescein monoglucoside and then to highly fluorescent fluorescein. Enzyme-m

Fluorescein di-β-D-glucuronide (FDGlcU) is colorless and nonfluorescent until it is hydrolyzed to the monoglucuronide and then to the highly fluorescent fluorescein.Beta-Glucuronidase (GUS) Assays, Cell Analysis, Enzyme and Protein Activity Assays, Flow

A fluorescein-labeled peptide substrate that is based upon the CREB ( cAMP responsive element binding) protein. The peptide substrate is phosphorylated by a wide range of Ser/Thr kinases.For use in combination with the LanthaScreen™ Tb-CREB [pSer133] ant

A general substrate for Ser/Thr kinases, the Crosstide peptide substrate is based upon GSK-3alpha/beta.For use in combination with the LanthaScreen™ Tb-pCrosstide Antibody (PV3564) to evaluate inhibitors in medium to high throughput screening application

A fluorescein-labeled peptide substrate based upon ezrin/radixin/moesin (ERM) that is phosphorylated by LRRK2 and LRRK2 mutants.For use in combination with the LanthaScreen™ Tb-anti-ERM (pLRRKtide) Antibody (PV4899) to evalute inhibitors in medium to hig

A fluorescein-labeled peptide substrate that is primarily used with the IkappaB kinase (IKK) family.For use in combination with the LanthaScreen™ Tb-IkappaBalpha [pSer32] antibody (PV3562) to evaluate inhibitors in medium to high throughput screening app

Inactive MAP2K1 (MEK1) has been labeled with fluorescein, and is a substrate for MAPK family members.For use in combination with the LanthaSreen™ Tb-MAP2K1 [pSer217/221] Antibody (PV4813) to evaluate inhibitors in medium to high throughput screening appl

A fluorescein-labeled peptide substrate based upon p53 containing Ser15. p53 is phosphorylated at Ser15 by a wide range of kinases including PI3K/AKT pathway kinases such DNA-PK, ATM, and ATR.For use in combination with the LanthaScreen™ Tb-anti-phospho-

A fluorescein-labeled peptide substrate for the Protein Kinase C (PKC) family.For use in combination with the LanthaScreen™ Tb-pSer (PKC Substrate) antibody (Cat. No. PV3560) to evaluate inhibitors in medium to high throughput screening applications.

A general fluorescein peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase activity.For use in combination with the LanthaScreen™ Tb-pY20 Antibody (PV3552) to evaluate inhibitors in medium to high t

4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).For use in combination with the LanthaScreen™ Tb-anti-4E-BP1 [pThr46] Antibody (Cat. No. PV4757) to evaluate inhibitors in medium to hi

A truncated version (residues 19-96) of ATF2 has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a substrate for the JNK and p38 families of kinases.For use in combination with the LanthaScreen™ Tb-anti-ATF2 [pThr71] antibody (P

EIF2A has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for the EIF2AK family of kinases including PERK (EIF2AK3) and PKR (EIF2AK2).For use in combination with the LanthaScreen™ Tb-anti-eIF2alpha [pSe

STAT1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for the Janus family of tyrosine kinases.For use in combination with the LanthaScreen™ Tb-anti-STAT1 [pTyr701] Antibody (PV4844) to evaluate inhibitors i

STAT3 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for the Janus family of tyrosine kinases.For use in combination with the LanthaScreen™ Tb-anti-STAT3 [pTyr705] Antibody (Cat. No. PV4846) to evaluate inh

Thermo Scientific™ Pierce beta-Galactosidase Precipitating Substrates is a chromogenic substrate for beta-galactosidase that is used to detect expression of vectors, plasmids, or DNA fragments that contain the reporter gene. X-Gal (5-bromo-4-chloro-3-ind

Alkaline phosphatase mediated hydrolysis of its phosphate substituent yields the blue-fluorescent 4-methylumbelliferyl (excitation/emission approx. 386/448nm).Alkaline Phosphatase Assays, Biochemical Toxicology Assays, Cell Analysis, Cellular Imaging, Ce

Selective protein kinase C (PKC) zeta inhibitor.Format/Formulation: Lyophilized trifluoroacetate salt, >97% purity by HPLC.Molecular Weight: 1928

NBT/BCIP Reagent Kit includes 1g samples of NBT (Nitro blue Tetrazolium Chloride) and BCIP (5-bromo-4-chloro-3-indolyl-1-phosphate)Cell Analysis, Cellular Imaging, IHC Staining and Detection, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohist

Actively respiring cells convert water-soluble Nitro blue tetrazolium chloride to a deep blue-colored precipitateCell Analysis, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cellular Imaging, IHC Staining and Detection, Imm

Novex™ AP Chemiluminescent Substrate is a non-radioactive ready-to-use solution of CDP-Star for chemiluminescence-based immunodetection of alkaline phosphatase (AP) on western blot or dot blot membranes. This reagent provides detection sensitivities supe

Novex™ AP Chromogenic Substrate is a ready to use single component reagent for sensitive immunodetection of alkaline phosphatase (AP) enzyme on western blot and dot blot membranes. Compatable for use with Western Breeze™ protocols, this ready-to-use solu