DNA Reagents

Perform fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature with Thermo Scientific™ Rapid DNA Ligation Kit. The kit contains T4 DNA ligase and a specially formulated 5X rapid ligation buffer optimized for fast and efficient DN

Like our first generation Seamless Cloning and Assembly Kit, GeneArt™ Seamless PLUS Cloning and Assembly Kit is the complete kit for simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in

Thermo Scientific™ Fermentas CloneJET PCR Cloning Kit is an advanced positive selection system for high efficiency cloning of PCR products generated with any thermostable DNA polymerase. Additionally, any other blunt or sticky-end DNA fragment can be clo

Invitrogen™ RNase T1, RNA-Grade cleaves 3′ of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites. Supplied in one tube of 200μL (1U/μL). R

Uses the pCR™4Blunt-TOPO™ vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. Include the tools necessary to clone and select recombinant vectors containing your PCR fragmen

aLICator LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in E. coli. The pLATE bacterial expression vectors are designed for high levels of target protein expressio

aLICator LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in E. coli. The pLATE bacterial expression vectors are designed for high levels of target protein expressio

aLICator LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in E. coli. The pLATE bacterial expression vectors are designed for high levels of target protein expressio

aLICator LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in E. coli. The pLATE bacterial expression vectors are designed for high levels of target protein expressio

aLICator LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in E. coli. The pLATE bacterial expression vectors are designed for high levels of target protein expressio

aLICator LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in E. coli. The pLATE bacterial expression vectors are designed for high levels of target protein expressio

Substrates include RNA, DNA, and oligonucleotides. One tube containing 500U (5U/μL) is provided along with 10X Reaction BufferThe enzyme can be used for tagging the 5′ ends of mRNA with oligonucleotides for mapping studies (RACE), 3′-end labe

Bac-to-Bac™ Baculovirus Expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombina

Bac-to-Bac™ Baculovirus Expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli (DH10Bac™) rather than homologou

Bac-to-Bac™ Baculovirus Expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombina

Fast DNA End Repair Kit is used for blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation.All components of the kit contain premixed reagents to reduce pipetting steps and provide convenience. The End Repair

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembl

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembl

GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembled us

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction. DNA fragments of different lengths are u

The inherent flexibility of this approach is suitable for small and large DNA constructs and includes both single and multiple inserts. The resulting products can be used for a variety of downstream applications including transformation, PCR and rolling

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction. DNA fragments of different lengths are u

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction. DNA fragments of different lengths are unifor

The GeneArt™ High-Order Genetic Assembly System is a highly efficient kit for the simultaneous and seamless assembly of up to 10 DNA fragments, totaling up to 110 Kbp in length, into any vector. The system relies on yeast’s ability to take up and r

The GeneArt™ High-Order Genetic Assembly System is a highly efficient kit for the simultaneous and seamless assembly of up to 10 DNA fragments, totaling up to 110 Kbp in length, into any vector. The system relies on yeast’s ability to take up and r

Offers chemical synthesis, cloning, and sequence verification of virtually any desired genetic sequence

GeneArt™ pYES1L Vector with Sapphire Technology™ is a ready-to-use, 9.3 Kb linearized BAC⁄YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt™ High-Order Genetic Assembly System (cat#A13285 & A13286). The vector has the capa

GeneArt™ Seamless Cloning and Assembly Enzyme Mix enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction. GeneArt™ Seamless enzyme

The GeneArt™ Seamless Cloning and Assembly Kit enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction. The kit contains everything

Features:Powerful—make substitutions, deletions, or insertions of up to 3 nucleotides in 1, 2, or 3 separate sites in DNA plasmids of up to 14kbFlexible—perform multi-site mutagenesis with degenerated nucleotides and single-site mutations up to 25 nucleo

The GeneArt™ Site-Directed Mutagenesis System provides a state-of-the-art, simple, convenient, and highly efficient means to generate mutations on a target construct in vitro in less than three hours. This system replaces the popular GeneTailor™ Site-Dir

The GeneArt™ Type IIs Assembly Kit uses a technology similar to Golden Gate cloning and can be used to assemble multiple fragments in a pre-determined order into any compatible vector. Since type IIs assembly is not based upon homologous recombination, t

The GeneArt™ Type IIs Assembly Kit uses a technology similar to Golden Gate cloning and can be used to assemble multiple fragments in a pre-determined order into any compatible vector. Since type IIs assembly is not based upon homologous recombination, t

The GeneArt™ Type IIs Assembly Kit uses a technology similar to Golden Gate cloning and can be used to assemble multiple fragments in a pre-determined order into any compatible vector. Since type IIs assembly is not based upon homologous recombination, t

The GeneRacer Kit is available with SuperScriptIII Reverse Transcriptase (RT) for improved amplification of the full-length 5' end from long and complex mRNA. The RNase H portion of SuperScriptIII RT has been mutated to avoid cleaving mRNA during cDNA sy

The GeneRacer Kit provides a method to obtain full-length 5' and 3' ends of cDNA using known cDNA sequence from expressed sequence tags (ESTs), subtracted cDNA, differential display, or library screening. RACE PCR products can be quickly and easily clone

The GeneRacer Kit is available with SuperScriptIII Reverse Transcriptase (RT) for improved amplification of the full-length 5' end from long and complex mRNA. The RNase H portion of SuperScriptIII RT has been mutated to avoid cleaving mRNA during cDNA sy

Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 nt) with 12 bp single-stranded complementary 5'-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by

Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 nt) with 12 bp single-stranded complementary 5'-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by

Thermo Scientific GeneJET Lysis Solution is a component of the GeneJET Plasmid Miniprep Kit (Cat. No. K0502, K0503) and may be purchased separately. Thermo Scientific GeneJET Lysis Solution is a component of the GeneJET Plasmid Miniprep Kit (Cat. No. K05

pBR322 is one of the most commonly used E.coli cloning vectors. HighlightsIsolated from E. coli (dam+, dcm+)More than 90% in the supercoiled formPurified by chromatography using proprietary patented technologyFor DNA sequence, sequence analysis, and map

Used as a Geneticin™ selectable expression plasmid for engineering stable cell linesFull length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cellsWPRE (woodchuck posttranscripti

Designed to create entry clone following PCR with attB-modified custom primersWith entry clone, recombine gene of interest into variety of expression vectors adapted for use in Gateway technologyPCR product is directionally cloned with efficiencies typic

Designed to create entry clone following PCR with attB-modified custom primersWith entry clone, recombine gene of interest into variety of expression vectors adapted for use in Gateway technologyPCR product is directionally cloned with efficiencies typic

Fast, 5-minute TOPO TA Cloning delivers 95% recombinants3'-T overhangs simplify cloning of Taq-amplified PCR productsFast growing MachI cells shorten cloning time Sequencing primer sites located close to cloning insertion site so sequences more insert, l

pCR™8 TOPO TA vector contains minimal MCS, so sequence more of insert and less of vectorPatented att sites make pCR™8 vector is simplest way to create an entry clone, which facilitates easy access via Gateway recombination cloning to variety of expressio

Contains minimal MCS, so sequence more of insert and less of vectorPatented att sites, pCR™8 vector to create entry clone, which facilitates easy access via Gateway recombination cloning to variety of expression vectors without tedious sub-cloning, savin

The pENTR™/D-TOPO™ Cloning Kits utilize a highly efficient, 5-minute cloning strategy (TOPO™ Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway™ System or the MultiSite Gateway™ System. Blunt-end PCR products

Blunt-end PCR products clone directionally at greater than 90% efficiency, with no ligase, post-PCR procedures, or restriction enzymes requiredRapidly shuttle cloned genes between multiple vector systemsReliable performanceFor access to Gateway System, P