Protein Extraction and Purification Reagents and Kits

An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturationAll B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal a

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin. The specially prepared nickel-NTA support consists of beaded agarose derivatized with th

Thermo Scientific™ NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The NE-PER Kit is a nuclear protein extraction method that inv

Thermo Scientific™ RIPA Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells. This RIPA buffer effectively lyses and extracts protein from cultured mammalian cells, including

Detergents and surfactants used to prepare protein and peptide samples can interfere with analysis by ELISA, isoelectric focusing, and mass spectrometry (MS). Removing detergents from peptide samples is especially challenging and critical for MS analysis

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin. The specially prepared nickel-NTA support consists of beaded agarose derivatized with th

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin. The specially prepared nickel-NTA support consists of beaded agarose derivatized with th

Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes. Isolation of mitochondria is typically a la

Thermo Scientific™ Pierce Protein L Agarose is a versatile, high-performance affinity resin for antibody purification that is available as bottled agarose beads, pre-packed spin columns, 96-well filter plates and complete IgG purification kits. Pierce Pr

Thermo Scientific™ Pierce IP Lysis Buffer is optimized for cell lysate yield, purity and compatibility with immunoprecipitation. The IP Lysis Buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS. This mod

Perform segregation and enrichment of proteins from five different cellular compartments with Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells. The kit enables the stepwise separation and extraction of cytoplasmic, membrane, nu

Perform mild extraction of total protein from tissue samples with Thermo Scientific™ T-PER Tissue Protein Extraction Reagent. This tissue cell lysis reagent utilizes a proprietary detergent in 25mM bicine and 150mM sodium chloride (pH 7.6) to maximize th

Thermo Scientific™ A549 (+hIGF-1) Control Lysate is a stimulated cell lysate that has been validated for the expression levels and phosphorylation of key targets in the AKT signaling pathway. Validated for the expression of AKT, PTEN, IRS1, IGF1-R, GSK3a

ActivX™ FP Probes feature a reactive fluorophosphonate group that specifically and covalently labels the active-site serine of enzymatically active serine hydrolases. These probes are available with a desthiobiotin (biotin analog) tag for selective enric

ActivX Desthiobiotin-ATP or ADP Probes are nucleotide derivatives that covalently modify the active site of enzymes at conserved lysine residues in the nucleotide binding site. The structure of these probes consists of a modified biotin (desthiobiotin) a

ActivX Desthiobiotin-ATP or ADP Probes are nucleotide derivatives that covalently modify the active site of enzymes at conserved lysine residues in the nucleotide binding site. The structure of these probes consists of a modified biotin (desthiobiotin) a

ActivX™ FP Probes feature a reactive fluorophosphonate group that specifically and covalently labels the active-site serine of enzymatically active serine hydrolases. These probes are available with a desthiobiotin (biotin analog) tag for selective enric

ActivX™ Desthiobiotin-GTP Probes contain a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. ActivX active-site enrichment probes are advantageous for determining active enzyme levels through the use of analog-tagged probes to a

The TAMRA-FP probe feature a reactive fluorophosphonate group that is used to label and detect active serine hydrolases by fluorescent gel imaging, capillary electrophoresis or mass spectrometry. An anti-TAMRA antibody is also available for immuno-enrich

Thermo Scientific™ AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines. AminoLink Coupling Resin is crosslinked 4

Thermo Scientific™ AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines. AminoLink Coupling Resin is crosslinked 4

Thermo Scientific™ AminoLink Plus Coupling Resin and Kits use aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines for preparation of affinity purification columns. The resins and kits use activate

Thermo Scientific™ AminoLink Plus Coupling Resin and Kits use aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines for preparation of affinity purification columns. The resins and kits use activate

Thermo Scientific™ AminoLink Plus Coupling Resin and Kits use aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines for preparation of affinity purification columns. The resins and kits use activate

AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups. Proteins and other molecules with primary amines can be covalently attached (immobilized) to the resin to make chromatography columns for use in affin

High sensitivity, freedom from background and long-wavelength excitability make these potential reagents for researcher, diagnostic and forensic applicationsResulting products of CBQCA are maximally excited at 450nm or by 442nm spectral line of He-Cd las

Recently, researchers described a simple method in which the ATTO-TAG FQ reagent was used to derivatize very dilute solutions (10-8 M) of peptides. Available as standalone product or as component of an amine-derivatization kit (A-2334). Particularly well

The ATTO-TAG FQ Plus Amine-Derivatization Kit is a comprehensive solution designed for the efficient and sensitive detection of primary amines. This kit is a modification of the ATTO-TAG FQ Amine Derivatization Kit (Cat. No. A2334), with a key improvemen

Perform efficient, gentle lysis and extraction of E.coli and other bacterial cells with Thermo Scientific™ B-PER Bacterial Protein Extraction Reagents. These easy-to-use cell lysis reagents are nonionic detergent solutions that effectively disrupt cells

Perform efficient, gentle lysis and extraction of E.coli and other bacterial cells with Thermo Scientific™ B-PER Bacterial Protein Extraction Reagents. These easy-to-use cell lysis reagents are nonionic detergent solutions that effectively disrupt cells

Perform efficient, gentle lysis and extraction of E.coli and other bacterial cells with Thermo Scientific™ B-PER Bacterial Protein Extraction Reagents. These easy-to-use cell lysis reagents are nonionic detergent solutions that effectively disrupt cells

Perform efficient, gentle lysis and extraction of E.coli and other bacterial cells with Thermo Scientific™ B-PER Bacterial Protein Extraction Reagents. These easy-to-use cell lysis reagents are nonionic detergent solutions that effectively disrupt cells

Validated for the expression of TP53 and phosphorylated TP53 by western blotting and mass spectrometry analysisOptimized - stimulation and lysis conditions optimized for detection of TP53 signaling targetsConvenient - lysate is ready-to-use for immunopre

Thermo Scientific™ CarboxyLink Coupling Resins and Kits provide for the covalent immobilization of peptides or other carboxyl-containing (-COOH) molecules to a porous, beaded resin for use in affinity purification procedures. These activated resins enabl

Thermo Scientific™ CarboxyLink Coupling Resins and Kits provide for the covalent immobilization of peptides or other carboxyl-containing (-COOH) molecules to a porous, beaded resin for use in affinity purification procedures. These activated resins enabl

The Thermo Scientific Pierce Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat.The Primary Cardiomyocyte Isolation Kit has be

Thermo Scientific™ Detoxi-Gel Endotoxin Removing Gel and Columns provide a quick method to remove endotoxins (lipopolysaccharides or LPS) from samples. Detoxi-Gel Resin is a beaded agarose support onto which polymixin B has been immobilized. This ligand

Thermo Scientific™ Detoxi-Gel Endotoxin Removing Gel and Columns provide a quick method to remove endotoxins (lipopolysaccharides or LPS) from samples. Detoxi-Gel Resin is a beaded agarose support onto which polymixin B has been immobilized. This ligand

The Thermo Scientific Pierce Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat.The Primary Cardiomyocyte Isolation Kit has be

Liver microsomes prepared from a pool of male Beagle Dogs.Total protein concentration: 20 mg/mlWe provide liver subcellular fractions from a variety of tox species, including human, nonhuman primates (Cynomolgus Monkey and Rhesus Monkey), dog (Beagle), r

This gentle and easy-to-use kit allows you to isolate flow-compatible human CD8+ T-cells from PBMC or directly from whole blood. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposi

The resin is conjugated with soybean trypsin inhibitor, which has a high affinity and binding capacity for enterokinaseThe enzyme catalytic site binds this agarose-based resin for simple batch or column-bound removal of EKMaxor other enterokinase prepara

The Thermo Scientific EZ-Link Desthiobiotinylation and Pull-Down Kit is a complete set of reagents to biotin-label purified proteins and use them to purify interaction complexes using streptavidin agarose resin with mild elution conditions.This all-in-on

Thermo Scientific™ Pierce Glutathione Agarose is a high-capacity, high-performance resin for affinity purification of GST-tagged fusion proteins from cellular lysates. The high–quality support consists of glutathione that has been immobilized by its cent

Thermo Scientific™ GlycoLink Immobilization Kits provide affinity columns and reagents to immobilize glycoproteins through oxidized sugar groups for use in affinity-purification applications or studies of protein-protein interactions. The GlycoLink Kits

The Thermo Scientific GlycoLink IP Kit provides components for effective immunoprecipitation (IP and co-IP) based on small-scale, covalent, affinity-resin immobilization of antibodies and other glycoproteins via oxidized sugar groups. The GlycoLink Kit u

Thermo Scientific™ GlycoLink Immobilization Kits provide affinity columns and reagents to immobilize glycoproteins through oxidized sugar groups for use in affinity-purification applications or studies of protein-protein interactions. The GlycoLink Kits

The Thermo Scientific Pierce Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat.The Primary Cardiomyocyte Isolation Kit has be

Validated for the expression RAS by western blotting and of NRAS, KRAS, and HRAS by mass spectrometry analysisOptimized - stimulation and lysis conditions optimized for detection of RAS signaling targetsConvenient - lysate is ready-to-use for immunopreci

Thermo Scientific™ HisPur Cobalt Resin is an immobilized metal chelate affinity (IMAC) support for specific, fast and gentle His-tagged purification.Additional Information and Online Ordering HisPur Cobalt Resins, Columns and Kits use a tetradentate chel