ELISA Substrates and Reagents

TMB (3,3',5,5' tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 nm and 652 nm. The color then changes to yellow (measured at 450 nm) upon addition of sulfu

Thermo Scientific™ Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers. OPD (o-phenylenediamine dihydrochloride) yields a water-soluble yellow-orange pr

Thermo Scientific™ Pierce ABTS Substrates are tablets and a ready-to-use solution of a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods. ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-di

Quantity: 65mL

Quantity: 1L

The CDP-Star™ Substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.This CDP-Star™ chemiluminescent substrat

Chemiluminescent substrates provide highly sensitive replacements for the widely used fluorogenic substrate methylumbelliferyl phosphate (MUP), and the colorimetric substrate p-nitrophenyl phosphate (pNPP). Low background luminescence coupled with high-i

Chemiluminescent substrates provide highly sensitive replacements for the widely used fluorogenic substrate methylumbelliferyl phosphate (MUP), and the colorimetric substrate p-nitrophenyl phosphate (pNPP). Low background luminescence coupled with high-i

This CSPD™ substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.This CSPD™ chemiluminescent substrate for a

Chemiluminescent substrates provide highly sensitive replacements for the widely used fluorogenic substrate methylumbelliferyl phosphate (MUP), and the colorimetric substrate p-nitrophenyl phosphate (pNPP). Low background luminescence coupled with high-i

Chemiluminescent substrates provide highly sensitive replacements for the widely used fluorogenic substrate methylumbelliferyl phosphate (MUP), and the colorimetric substrate p-nitrophenyl phosphate (pNPP). Low background luminescence coupled with high-i

Chemiluminescent substrates provide highly sensitive replacements for the widely used fluorogenic substrate methylumbelliferyl phosphate (MUP), and the colorimetric substrate p-nitrophenyl phosphate (pNPP). Low background luminescence coupled with high-i

When combined with DynaLight Trigger Solution, DynaLight Substrate with RapidGlow Enhancer delivers maximum signal within seconds. The environmentally-friendly DynaLight Trigger Solution provides maximum signal within 1 second of addition, with a longer

Reagent sold ready-to-use.

Buffer Kit for Antibody Pairs is for use with Invitrogen Antibody Pairs. Kit provides sufficient materials for ten ELISA plates.Build Your Own Immunoassay, Cell Analysis, Immunoassays

ELISA Carbonate Coating Buffer is intended for use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA.The buffer is a 50 mM carbonate buffer, pH 9.4.

ELISA Phosphate Coating Buffer is intended for use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA.The buffer is a 10 mM phosphate buffer, pH 7.4.

ELISA Stop Solution is a ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution. As TMB substrate solution reacts with horseradish peroxidase, it produces a pronounced bl

Type: Reagent Format: 30X Concentrate

For use with Galacto-Light Plus Reporter Gene Assay System.

The Galacton-Plus™ Substrate is a concentrated chemiluminescent substrate for E.coli β-galactosidase assays.For Research Use Only. Not for use in diagnostics procedures.

Galacton-Star substrate is a chemiluminescent substrate designed for the rapid, and sensitive detection of beta-galactosidase in cell lysates. The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric

Sapphire-II chemiluminescence enhancers, are essential components of tube or microplate-based assays, significantly reducing the effect of aqueous quenching. Enhancers also tune the wavelength of light emission. Sapphire-II enhancer shifts the light emis

Thermo Scientific™ Pierce ONPG Substrate is the preferred colorimetric substrate for ELISA applications involving β-galactosidase (β-Gal) as the reporter enzyme. ONPG (ω-nitrophenyl-β-D-galactopyranoside) is the preferred colorimetric substrate for ELISA

Thermo Scientific™ Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers. OPD (o-phenylenediamine dihydrochloride) yields a water-soluble yellow-orange pr

Thermo Scientific™ Pierce Substrate Buffers include optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection. The Stable Peroxide Substrate Buffer is an ideal for

Thermo Scientific™ Pierce Substrate Buffers include optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection. The Stable Peroxide Substrate Buffer is an ideal for

PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product abs

Sufficient materials are provided to run 10 assays using 96-well plates. The kit does not include the following items: qPCR plates, cold blocks, and pipette tips.

Thermo Scientific™ QuantaBlu Fluorogenic Peroxidase Substrate is a soluble fluorogenic (chemifluorescent) substrate for detection of peroxidase activity in ELISA methods.Additional Information and Online Ordering In the presence of active peroxidase the

Thermo Scientific™ QuantaBlu Fluorogenic Peroxidase Substrate is a soluble fluorogenic (chemifluorescent) substrate for detection of peroxidase activity in ELISA methods.Additional Information and Online Ordering In the presence of active peroxidase the

Thermo Scientific™ QuantaRed Enhanced Chemifluorescent HRP Substrate is the most sensitive fluorescent ELISA substrate, providing sensitivity comparable to enhanced chemiluminescence. QuantaRed Enhanced Chemifluorescent HRP Substrate enables high-yield a

Thermo Scientific™ SuperSignal ELISA Femto Maximum Sensitivity Substrate is formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications. SuperSignal ELISA Femto Substrate generates detectable chemiluminescence (

Thermo Scientific™ SuperSignal ELISA Pico Chemiluminescent Substrate is optimized to generate an intense light signal and provide exception performance in luminometer-based assays. SuperSignal ELISA Pico Substrate provides chemiluminescent detection of h

The Emerald-II chemiluminescence enhancer significantly reduces aqueous quenching and tunes the wavelength of light emission. • Aqueous environments reduce chemiluminescent signal intensity of 1,2-dioxetane substrates by water-induced quenching; chemilum

Thermo Scientific™ Pierce Stop Solution for TMB is 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB). TMB Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3«,5,5«-tetramethylben