Gene Reporter Systems

The DNAclear cDNA Purification Kit is designed to purify double-stranded DNA (dsDNA) generated by enzymatic reactions such as cDNA synthesis and PCR. The process is simple and fast. DNA amounts ranging from 1ng to 3μg can be efficiently purified and conc

The Alexa Fluor™ reactive dye decapack is specially packaged succinimidyl ester dye for labeling of DNA or RNA samples that have been modified by incorporation of aminoallyl-dUTP (Cat. No. A21664) or aminohexylacrylamido-dUTP (Cat. No. A32761). Every pac

The Alexa Fluor™ 555 and 647 Reactive Dye Decapack is optimized for use with aminoallyl labeling techniques. The single-use packaging of the reactive dyes eliminates the need to aliquot bulk dyes, providing convenience and ensuring maximum activity of th

The Alexa Fluor 555 Reactive Dye Decapack is optimized for use with aminoallyl labeling techniques. The single-use packaging of the reactive dye eliminates the need to aliquot bulk dyes, providing convenience and ensuring maximum activity of the dyes. Th

The Alexa Fluor™ 555 dyes, used to label nucleotides, are compatible with commonly used microarray scanners, and provide greater signal correlation (R2) values than the spectrally similar Cy™3 and Cy5 dye pair, improving the resolution of two-color micro

Labeled 5-aminohexylacrylamido-dUTP (aha-dUTP) nucleotides are modified at the C-5 position of uridine with a unique hexylacrylamide linker, which serves as a spacer between the nucleotide and the dye. This spacer reduces interactions between the nucleot

The Alexa Fluor™ 647 Reactive Dye Decapack is optimized for use with aminoallyl labeling techniques. The single-use packaging of the reactive dye eliminates the need to aliquot bulk dyes, providing convenience and ensuring maximum activity of the dyes. T

The Alexa Fluor™ 647 dyes, used to label nucleotides, are compatible with commonly used microarray scanners, and provide greater signal correlation (R2) valuesthan the spectrally similar Cy™3 and Cy5 dye pair, improving the resolution of two-color microa

Labeled 5-aminohexylacrylamido-dUTP (aha-dUTP) nucleotides are modified at the C-5 position of uridine with a unique hexylacrylamide linker, which serves as a spacer between the nucleotide and the dye. This spacer reduces interactions between the nucleot

Improved first- and second-strand cDNA synthesisIncluded in the kit is ArrayScript™ RT, a rationally engineered M-MLV reverse transcriptase, which produces equivalent or higher yields of full-length cDNA compared to other enzymes. The second-strand cDNA

When hybridized to complementary spots on a gene array, ArrayControl RNA Spikes can be used to monitor the efficiency of reverse transcription and aRNA amplification, labeling, and hybridization. They can also be used to assess the precision, accuracy, a

Supports a flexible solution to your genomic labeling needs. Part of the BioPrime Plus Array CGH Genomic Labeling System. Using theBioPrime Plus Array CGH Genomic Labeling Systems, you can expect:––High yields of fluorescently labeled genomic DNA–signal-

Using the BioPrime Plus Array CGH Genomic Labeling System, you can expect:––High yields of fluorescently labeled genomic DNA–signal-to-noise ratios–detection of gene copy number variations.These systems combine a highly concentrated exo-Klenow fragment o

Biotin-14-CTP can be efficiently incorporated into RNA transcripts using T7, SP6, or T3 RNA polymerase and the appropriate template in the presence of ATP, GTP, UTP, and Biotin-14-CTP. The biotinylated RNA can be used as a probe in membrane-based or in s

About Biotin-14-dCTPBiotin-14-dCTP is a patented dCTP analog with biotin attached at the N4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, dGTP, a

A novel cloning process places an approx. 50-bp DNA oligonucleotide immediately following a U6 pol III promoter into the BLOCK-iT U6 entry vector. After efficient recombination of the entry vector into the pAd/BLOCK-iT-DEST vector, followed by viral prod

The BLOCK-iT™ Pol II miR RNAi Expression Vector Kits combine the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same

The BLOCK-iT™ Pol II miR RNAi Expression Vector Kits combine the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same

Broadens the potential RNAi applications beyond those of other traditional retroviral systems (Naldini, 1998). The BLOCK-iT Lentiviral Pol II miR RNAi Expression System includes:o A BLOCK-iT Pol II miR RNAi Expression Vector Kit for production of an expr

Broadens the potential RNAi applications beyond those of other traditional retroviral systems (Naldini, 1998). The BLOCK-iT Lentiviral Pol II miR RNAi Expression System with EmGFP includes: o A BLOCK-iT Pol II miR RNAi Expression Vector Kit for productio

A novel cloning process places an approx. 50-bp DNA oligonucleotide immediately following a U6 pol III promoter into the BLOCK-iT U6 entry vector. The oligonucleotide is designed to express RNA that forms a stemLoop structure containing the sense and ant

A novel cloning process places an approx. 50-bp DNA oligonucleotide immediately following a U6 pol III promoter into the BLOCK-iT U6 entry vector. The oligonucleotide is designed to express RNA that forms a stemLoop structure containing the sense and ant

The BLOCK-iT Lentiviral RNAi Zeo Gateway Vector Kit contains the pLenti4/BLOCK-iT-DEST expression vector which enables lentiviral delivery and genomic integration of DNA coding for shRNA. Once expressed, the shRNA is processed by cellular machinery and i

The pcDNA™6.2-GW/miR vector included in the BLOCK-iT Pol II miR RNAi Expression Vector Kit is designed to express artificial miRNAs which are engineered to have 100% homology to your target sequence and will result in target cleavage. This vector include

The pcDNA™6.2-GW/EmGFP-miR vector included in the BLOCK-iT Pol II miR RNAi Expression Vector Kit with EmGFP is designed to express artificial miRNAs which are engineered to have 100% homology to your target sequence and will result in target cleavage. Th

An easy cloning process places an approx. 50-bp oligonucleotide DNA immediately following a U6 pol III type promoter. Expression of this RNAi cassette forms a shRNA molecule in the cell that will be processed and act as short interfering RNA (siRNA) that

This vector expresses the next-generation Aequorea victoria EGFP gene, encoding the emerald green fluorescent protein (EmGFP). The cell will express the EmGFP, which is bright, photostable, and ideal for testing transduction. The CytoTune™ EmGFP Sendai F

The DNAclear™ cDNA Purification Kit process is simple and fast. DNA amounts ranging from 1ng to 3μg can be efficiently purified and concentrated. The DNAclear procedure consists of three steps: 1) DNA is bound to the Microfilter Cartridge in the presence

The FluoReporter™ lacZ Flow Cytometry Kit provides materials and protocols for quantitating β-galactosidase activity with fluorescein di-V-galactoside (FDG) in single cells using flow cytometry. This kit is accompanied by a license to practice patented t

The FluoReporter™ lacZ/Galactosidase Quantitation Kit includes the β-galactosidase substrate, 3-carboxyumbelliferyl β-D-galactopyranoside that produces the blue-fluorescent hydrolysis product, 7-hydroxycoumarin-3-carboxlic acid.Beta-Galactosidase Assays,

Glow assay kinetics provides a window during which measurements may be performed, facilitating HTS applications where assay automation is usedCan be used with either mammalian or yeast model systems, providing flexibility in choice of model systemsWide d

Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram rangeAssay sensitivity is 100- to 1000-fold better than with either isotopic/non-isotopic assays for chloramphenicol acetyl transferase (

Homogeneous assay format allows detection of β-galactosidase in presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possibleThe kinetics of glow-a

Gal-Screen homogeneous assay is ideally suited for screening applications where assay automation is required. The Gal-Screen system uses Galacton-Star™ chemiluminescent substrate for convenient measurement in a luminometer. Light emission reaches maximum

The Ambion™ GLOBINclear™-Mouse/Rat Kit employs a novel, non-enzymatic technology that rapidly depletes >95% of the alpha and beta globin mRNA from total RNA preparations derived from whole blood. The kit includes sufficient reagents for 20 reactions.• Re

The GLOBINclear-Human Kit is a rapid and robust treatment for RNA derived from human whole blood that uses a novel, non-enzymatic technology to rapidly deplete >95% of the alpha and beta globin mRNA from total RNA preparations.Advantages of the GLOBINcle

Human Cot-1 DNA is commonly used to block nonspecific hybridization in microarray screening. It can also be used to suppress repetitive DNA sequences for the direct mapping of human DNA or mapping genomic clones to panels of somatic-cell hybrids for chro

Human Cot-1 DNA-Fluorometric QC is placental DNA predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences, such as the Alu and Kpn family members (1,2). Quantifying Cot-1 DNA™ by fluorometry provides more accurate concentration data o

The ImaGene Green™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12FDG. Once inside the cell, the substrates are cleaved by β-galactosidase, prod

The ImaGene Red™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12RG. Once inside the cell, the substrates are cleaved by β-galactosidase, produci

Form: LiquidFormat: BottleSubstrate: Alkaline Phosphatase

Homogeneous assay format allows detection of firefly luciferase in presence of normal culture media without removal of media and without an additional cell lysis step, providing easiest and most streamlined assay procedure possibleGlow assay kinetics pro

System overviewExpression profiling using gene arrays has become the most widely used method for assessing gene expression on a genome-wide/scale. The primary limitation of this technology is the requirement for large amounts of RNA for array hybridizati

Please see our newest bisulfite conversion kit, Cells-to-CpG™ Bisulfite Conversion System, designed to provide more flexibility in starting samples without the need to purify your DNA and more confidence with available methylation controls. Cells-to-CpG™

Mouse Cot-1 DNA™ is commonly used to block non-specific hybridization in microarray screening. It can also be used to suppress hybridization of rodent repetitive DNA sequences when mapping rodent clones by in situ hybridization or Southern blotting and t

Sensitive detection of alkaline phosphatase reporter enzyme enables a large number of applications in many areas of life science research, including gene expression, viral function assays, vaccine development, development of viral vectors and gene delive

Detection of secreted reporter permits analysis without destroying valuable cellsWide dynamic range of alkaline phosphatase assay lets user measure enzyme level accurately from femtogram to nanogram rangeAssay sensitivity is 100- to 1,000-fold better tha

This vector contains elements that allow packaging of the construct into lentiviral particles and the Blasticidin resistance marker for selection of stable cell lines. The pLenti6/TR vector is designed for use with the BLOCK-iT™ Inducible H1 Lentiviral R

The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression. This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT Luciferase miRNA Expression Repor

Produces probes that can detect nucleic acids on northern or Southern blots, plaque lifts, colony hybridizations, and in situ hybridizationYields >109cpm/μg control DNA using [32P]-dCTPLabels 25ng of DNA in one reactionPerformance and Quality Testing: In