Modifying Enzymes and Buffers

Thermo Scientific Exonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact. It does not cleave DNA strands with terminal 3'

Kit contains reagents for efficient, complete digestion of DNA along with removal of enzyme and divalent cations post-digestionHyperactive TURBO DNase is catalytically superior enzyme compared to wild type DNase IRemoves trace quantities of DNA that can

RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions. Does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and HPerforms under a wide ra

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.The enzyme activity is strictly dep

Thermo Scientific FastAP Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA, and nucleotides. This enzyme also removes phosphate groups from proteins.FastAP is a novel alkaline phosphatase, which is ac

RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues.It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an ad

Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a te

Cleaves double-stranded DNA nonspecifically to leave 5' phosphorylated oligodeoxynucleotides with an increased affinity for DNA-binding and remains active in presence of salt.Up to 50X more activity and 350% greater catalytic efficiencyEfficiently degrad

Catalyzes the in vitro recombination of PCR products or subcloning DNA segments from clones (containing attB sites) and a donor vector (containing attP sites) to generate entry clonesContains enzymes and buffer in a single mix to enable convenient ten-mi

Thermo Scientific Rnase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues (see Figure 1 in Supporting Data). It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleot

Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also

The 5X Kinase Buffer A is a concentrated general kinase assay buffer that can be used with a variety of assay technologies such as, LanthaScreen™, Adapta™, and Z’-LYTE™ assays.Ready to use and validated with LanthaScreen™, Adapta™, and Z’LYTE

An improved version of Tobacco Etch Virus (TEV) AcTEV™ Protease is highly site-specific, highly active and significantly more stable than native TEV protease, resulting in enhanced long-term activity.Highly specific cleavage activityEnhanced enzyme stabi

Agarase specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides. Agarase allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose. The recovered nucleic acids can be directly used for amp

DNase I functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with 5'-phosphate and 3'-hydroxyl group. RNase-free DNase I is recommended to degrade DNA in presence of RNA, in absence of RNase is critical to maintain integr

For large batch sizes, use of RNase is still only effective technique applicable to remove host RNA. Other methods such as selective precipitation, gel filtration, chromatography, and endogenous E. coli RNase digestion have only been effective for small-

Ambion RNase A is endonuclease that specifically cleaves 3' of U and C residues. Affinity Purified RNase A is intended for critical applications when absence of DNase and other nonspecific nuclease activities is essential.Supplied in one tube containing

Ribonuclease H (RNase H) is isolated from an E. coli strain that over-expresses the gene. RNase H specifically degrades RNA in RNA:DNA hybrids to produce 3'-hydroxyl and 5'-phosphate terminated products.Will not degrade DNA or unhybridized RNAIntegral pa

RNase I degrades all RNA dinucleotide bonds leaving a 5' hydroxyl and 2', 3' cyclic monophosphate. RNase I will degrade any RNA to mixture of mononucleotides, dinucleotides, and trinucleotides and does not degrade DNA, although it will bind to DNA. It ha

Escherichia coli Ribonuclease III (RNase III; EC 3.1.24) is specific endoribonuclease. In E. coli, RNase III cleaves ribosomal RNA (rRNA) precursors during maturation of rRNA. Enzyme cleaves dsRNA into 12–15 bp dsRNA fragments with 2 to 3 nucleotide 3' o

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. The Invitrogen Anza Restriction Enzyme Cloning System utilizes a single buffer system for complete digestions and incorporates Anza cloning enzy

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. The Invitrogen Anza Restriction Enzyme Cloning System utilizes a single buffer system for complete digestions and incorporates Anza cloning enzy

The Invitrogen™ Anza™ DNA End Repair Kit is used to convert DNA with cohesive ends to blunt-ended 5’-phosphorylated DNA for use in blunt-end ligation reactions. The included pre-mixed Anza™ DNA End Repair Mix contains T4 DNA polymerase, Klenow Frag

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. The Invitrogen Anza Restriction Enzyme Cloning System utilizes a single buffer system for complete digestions and incorporates Anza cloning enzy

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. The Invitrogen Anza Restriction Enzyme Cloning System utilizes a single buffer system for complete digestions and incorporates Anza cloning enzy

BAP is active at 65°C for at least 1 hr. and is inactivated by phenol extractionSource: Purified from E. coli C90Performance and Quality Testing: Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency determine

Bovine Serum Albumin is suitable for use as enzyme stabilizer during purification or for dilution of restriction endonucleases and nucleic acid modifying enzymes. BSA is also commonly used in DNA and protein labeling experiments as a blocking agent to mi

Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance an

Concentration: 20 units/μLSource: Purified from calf intestinal mucosaPerformance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assayUnit Definition

Concentration: 20 units/μLSource: Purified from calf intestinal mucosaPerformance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assayUnit Definition

These Control Vesicles are insect-derived purified plasma membranes with no inserted transport proteins for use as a negative control in vesicular transport assays. Our Control Vesicles control for nonspecific absorption and transport of test substrates

CorrectASE™ enzyme removes mismatches caused by oligonuceotide synthesis errors, leading to a 3–10 fold reduction in mutations in your synthetic genes or fragments.By introducing CorrectASE™ enzyme into your do-it-yourself gene syntheis workflow, you can

The enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5'-CpG-3' context.M.SssI is specifically formulated for fast reaction times without compromising the

Cytochrome P450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells infected with recombinant baculovirus containing a human CYP450 isozyme, as well as human cytochrome P450 reductase. For this particular isozyme (CYP2C9), human cytochro

Cytochrome P450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells infected with recombinant baculovirus containing a human CYP450 isozyme, as well as human cytochrome P450 reductase.Single human P450 isozyme for detailed drug metabolis

Cytochrome P450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells infected with recombinant baculovirus containing a human CYP450 isozyme, as well as human cytochrome P450 reductase. For this particular isozyme (CYP3A4), human cytochro

DNA-free™ DNase treatment and removal reagents are designed for removal of contaminating DNA from RNA samples and for removal of DNase after treatment.No organic extraction or heat inactivation requiredIncludes novel reagent to remove DNaseRecombinant DN

DNase I (Deoxyribonuclease I) digests single- and double-stranded DNA to oligodeoxyribonucleotides containing a 5' phosphateRibonuclease has been reduced to nondetectable levelsSpecific activity of DNase I is typically in range of 10,000–25,000 units/mgF

DNase I, Amplification Grade, digests single- and double-stranded DNA to oligodexyribonuleotides containing a 5' phosphate. DNase I, Amplification Grade, is suitable for eliminating DNA during critical RNA purification procedures such as those prior to R

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.The enzyme activity is strictly dep

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.The enzyme activity is strictly dep

Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. The enzyme is not active on blunt-ended DNA.FeaturesActivity in wide

Single-stranded nucleic acids are not substrates for this enzyme.Source: Purified from E. coli 594 (Su-) bearing λ lysogen gt4lop-11 lig+ S7Performance and Quality Testing: Endodeoxyribonuclease and 3' and 5' exodeoxyribonuclease assays; ligation efficie

Endonuclease IV (Endo IV) recognizes apurinic/apyrimidinic (AP) sites of dsDNA and cleaves the phosphodiester bond 5' to the lesion generating a hydroxyl group at the 3'-terminus. The enzyme can also act as a 3'-diesterase that is able to release 3'-phos

Endonuclease V, T. maritima (Endo V) is a 3'-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine (see Figure 1).Endonuclease V is also active toward abasic sites

Thermo Scientific EpiJET Bisulfite Conversion Kit is designed for simple and reliable bisulfite conversion of DNA for methylation analysis. In the bisulfite reaction all unmethylated cytosines are deaminated and converted to uracils, while methylated cyt

5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression.EpiJET DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyze DNA methylation status at a

Exonuclease III (ExoIII) exhibits four catalytic activities (see Reference1). The 3'≥5' exodeoxyribonuclease activity of ExoIII is specific for double-stranded DNA. ExoIII degrades dsDNA from blunt ends, 5'-overhangs or nicks, releases 5'-mononucleotides

SuperScript™ IV VILO™ (SSIV VILO) Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. Inclusion of ezDNase, further accelerates the RT-qPCR workflow through an extremely simplified ge