Cell Function Reagents and Kits

Used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixationKit has been optimized and validated for use wit

PrestoBlue and PrestoBlue HS cell viability reagents are ready-to-use, nontoxic, resazurin-based solutions that function as long term kinetic cell health indicators for fluorescence and absorbance-based microplate assays. These reagents are cell permeabl

CellTrace™ Violet Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Superior performance—bright, single-peak staining enables visualization of multiple generations Long-term signal stability—well-retained in cells for several days post stain Versatile—multiple colors available to easily combine with antibodies or markers of cell function, such as GFP Simple, robust staining protocol View a selection guide for all CellTrace™ Cell Proliferation Kits for flow cytometry. Superior fluorescent staining Successful proliferation analysis by dye dilution (see figure below) requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTrace™ Violet dye enables the visualization of ten or more generations of proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluor

Since the amount of DNA in cells is highly regulated, methods to detect DNA content offer improved accuracy over metabolism-based cellular proliferation or cytotoxicity assays, which can be affected by cell changes that are unrelated to cell number. CyQU

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. Fluo-3 has been used to image the spatial dynamics of Ca2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second

The LIVE/DEAD BacLight Bacterial Viability Kits are convenient and easy-to-use kits for monitoring the viability of bacterial populations based on the membrane integrity of the cell. These kits provide a two-color fluorescence assay of bacterial viabilit

Used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixationKit has been optimized and validated for use wit

CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.• Superior performance—bright, single-peak staining enables visualization of multiple generations

CyQUANT MTT and XTT Cell Viability assays are colorimetric microplate assays used to measure the metabolic activity of cells, which is an indicator of cell viability, proliferation, and cytotoxicity. CyQUANT MTT and XTT function as cell health indicators

MTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. The formazan is then solubilized and its concentration determined by optical density.

Although selectivity of PBFI for K+ is less than that of calcium indicators such as fura-2, it is sufficient for detection of physiological concentrations of K+ in presence of other monovalent cationsSpectral response of PBFI upon ion binding permit exci

In the Vybrant™ Cell Metabolic Assay Kit, nonfluorescent C12-resazurin is reduced to fluorescent C12-resorufin by viable cells. The resulting signal is proportional to the number of cells present. C12-resazurin has better cellular retention than alamarBl

Essentially nonfluorescent in water, only becoming appreciably fluorescent when bound to membranesCell Analysis, Cell Structure, Membranes (General) and Lipids

2-NBDG is a fluorescent glucose analog that has been used to monitor glucose uptake in live cells, as an indicator of cell viability. Although sensitive to its environment NBD fluorescence typically displays excitation/emission maxima of ∼465/540 nm and