Cell Function Reagents and Kits

Used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixationKit has been optimized and validated for use wit

PrestoBlue and PrestoBlue HS cell viability reagents are ready-to-use, nontoxic, resazurin-based solutions that function as long term kinetic cell health indicators for fluorescence and absorbance-based microplate assays. These reagents are cell permeabl

CyQUANT MTT and XTT Cell Viability assays are colorimetric microplate assays used to measure the metabolic activity of cells, which is an indicator of cell viability, proliferation, and cytotoxicity. CyQUANT MTT and XTT function as cell health indicators

CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.• Superior performance—bright, single-peak staining enables visualization of multiple generations

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. Fluo-3 has been used to image the spatial dynamics of Ca2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second

The LIVE/DEAD BacLight Bacterial Viability Kits are convenient and easy-to-use kits for monitoring the viability of bacterial populations based on the membrane integrity of the cell. These kits provide a two-color fluorescence assay of bacterial viabilit

alamarBlue and alamarBlue HS cell viability reagents are ready-to-use, non-toxic, solutions based on resazurin. These reagents function as cell health indicators, enabling quantitative measurement of cell viability and proliferation using absorbance- or

CellTrace™ Violet Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Superior performance—bright, single-peak staining enables visualization of multiple generations Long-term signal stability—well-retained in cells for several days post stain Versatile—multiple colors available to easily combine with antibodies or markers of cell function, such as GFP Simple, robust staining protocol View a selection guide for all CellTrace™ Cell Proliferation Kits for flow cytometry. Superior fluorescent staining Successful proliferation analysis by dye dilution (see figure below) requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTrace™ Violet dye enables the visualization of ten or more generations of proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluor

MTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. The formazan is then solubilized and its concentration determined by optical density.

Although selectivity of PBFI for K+ is less than that of calcium indicators such as fura-2, it is sufficient for detection of physiological concentrations of K+ in presence of other monovalent cationsSpectral response of PBFI upon ion binding permit exci

ReadyProbes™ Cell Viability Imaging Kit, Red/Blue contains NucBlue™ Live reagent (Hoechst 33342) and propidium iodide. Just add 2 drops of each stable reagent (at room temperature) to 1mL of cell growth media, then determine viability by counting total v

Tetramethylrhodamine, ethyl ester (TMRE) is a cell-permeant, cationic, red-orange fluorescent dye that is readily sequestered by active mitochondria.Dissolve the dye in high-quality anhydrous DMSO or ethanol to prepare stock concentration at 1 to 10 mM.

In the Vybrant™ Cell Metabolic Assay Kit, nonfluorescent C12-resazurin is reduced to fluorescent C12-resorufin by viable cells. The resulting signal is proportional to the number of cells present. C12-resazurin has better cellular retention than alamarBl

Essentially nonfluorescent in water, only becoming appreciably fluorescent when bound to membranesCell Analysis, Cell Structure, Membranes (General) and Lipids

2-NBDG is a fluorescent glucose analog that has been used to monitor glucose uptake in live cells, as an indicator of cell viability. Although sensitive to its environment NBD fluorescence typically displays excitation/emission maxima of ∼465/540 nm and

Measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent productUpon hydrolysis by intracellular esterases, this AM ester also yields

Exhibits significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectivelyThis pH dependence allows ratio of fluorescence intensities from dye at two emission wavelengths, typically 580nm

Exhibits significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectivelyThis pH dependence allows ratio of fluorescence intensities from the dye at two emission wavelengths, typically 58

Measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent productElectronically neutral AM ester of CFDA can be loaded into cells at lo

6-NBDG is a fluorescent nonhydrolyzable glucose analog that has been used to monitor glucose uptake and transport in live cells. Although sensitive to its environment NBD fluorescence typically displays excitation/emission maxima of ∼465/540 nm and can b

Alkaline Phosphatase Live Stain is a stem cell imaging product that allows users to differentially stain pluripotent stem cells (PSCs). The dye is a cell-permeable fluorescent substrate for alkaline phosphatase (AP) that is non-toxic to cells, diffusing

The Amplex Red Acetylcholine/Acetylcholinesterase Assay Kit provides an ultrasensitive method for detecting acetylcholinesterase (AChE) activity in a fluorescence microplate reader or fluorometer. AChE activity is monitored indirectly using 10-acetyl-3,7

The Amplex Red and Amplex UltraRed reagents are highly sensitive and stable fluorescent probes for the detection of hydrogen peroxide (H2O2) and ideal fluorogenic substrates for peroxidase. These reagents can be used for solution-based assays to detect t

Provides sensitive and simple fluorometric method for detecting as little as 50mU/mL of catalase activity in purified system in 100μL assay volumeDrug Discovery and Development, Enzyme and Protein Activity Assays, Oxidase and Peroxidase Activity, Protein

Designed for minimal autofluorescence interference, the Amplex Red Cholesterol Assay Kit provides a sensitive, rapid, and simple fluorometric method for detecting very low concentrations of cholesterol using a fluorescence microplate reader or fluoromete

The Amplex Red Galactose/Galactose Oxidase Assay Kit provides a sensitive and simple method for detecting galactose oxidase activity or galactose using a fluorescence microplate reader or fluorometer. Galactose oxidase is an important tool in the detecti

The Amplex Red Glucose/Glucose Oxidase Assay Kit provides a sensitive and simple method for detecting d-glucose or glucose oxidase using a fluorescence microplate reader or fluorometer. The Amplex Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) is a col

The Amplex Red Glutamic Acid/Glutamate Oxidase Assay Kit provides a sensitive and simple method for detecting glutamic acid/glutamate oxidase activity using a fluorescence microplate reader or fluorometer. The Amplex Red Glutamic Acid/Glutamate Oxidase A

Assay uses AmplexRed reagent (10-acetyl-3,7-dihydroxyphenoxazine) to detect hydrogen peroxide (H2O2) or peroxidase activity in vitro. AmplexRed reagent, in combination with horseradish peroxidase (HRP), can detect H2O2 released from biological samples, i

Provides a sensitive and simple fluorometric method for detecting monoamine oxidase activity in purified enzyme preparations at levels as low as 12μU/mL and in tissue samples with as little as 20μg protein in a 100μL assay volume.Enzyme and Protein Activ

The Amplex Red Neuraminidase (Sialidase) Assay Kit provides a sensitive and simple method for detecting neuraminidase activity in serum and in purified enzyme systems using either a fluorescence microplate reader or fluorometer. Neuraminidase (also known

The Amplex Red Phosphatidylcholine-Specific Phospholipase C Assay Kit provides a sensitive method for detecting phosphatidylcholine-specific phospholipase C (PC-PLC) activity using a fluorescence microplate reader or fluorometer. In this enzyme-coupled a

The Amplex Red Phospholipase D Assay Kit provides a sensitive and simple method to detect Phospholipase D (PLD) activity using a fluorescence microplate reader or fluorometer. PLD activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine

The Amplex Red Sphingomyelinase Assay Kit provides a sensitive, rapid, and simple fluorometric method for detecting very low concentrations of sphingomyelinase using a fluorescence microplate reader or fluorometer. In this enzyme-coupled assay, sphingomy

The Amplex Red Uric Acid/Uricase Assay Kit provides a sensitive and simple method for detecting uric acid or uricase using either a fluorescence microplate reader or fluorometer. Serum uric acid is the end product of purine metabolism in the body tissues

Amplex Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. enic substrates and assay kits. It is designed to terminate reactions containing u

The ATP Determination Kit is a highly sensitive luminescence assay designed for the quantitative detection of ATP levels in biochemical and cell viability assays. This ATP assay kit utilizes the reaction between recombinant firefly luciferase and its substrate D-luciferin and is based on luciferase’s absolute requirement for ATP in producing light (emission maximum approximately 560 nm at pH 7.8). With its exceptional sensitivity, capable of detecting as little as 0.1 picomole of ATP, this assay is well-suited for kinetic studies and can be used with luminometers or microplate readers equipped with a luminescence mode, such as the Thermo Scientific Varioskan ALF and LUX Multimode Microplate Readers. This luciferase ATP assay includes sufficient reagents for ten 96-well plates and offers a unique advantage over other commercially available ATP detection kits by providing the luciferase and luciferin components separately, allowing researchers to optimize the reaction conditions fo

The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cell

The BacLight Green (Cat. no. B-35000) and BacLight Red (Cat. no. B-35001) bacterial stains are fluorescent labeling reagents for detecting and monitoring bacteria. These two dyes are not nucleic acid stains. Bacteria stained with the BacLight Green and B

The BacLight Green (Cat. no. B-35000) and BacLight Red (Cat. no. B-35001) bacterial stains are fluorescent labeling reagents for detecting and monitoring bacteria. These two dyes are not nucleic acid stains. Bacteria stained with the BacLight Green and B

The BacLight™ RedoxSensor™ CTC Vitality Kit provides effective reagents for evaluating bacterial cell health and vitality that can withstand fixation procedures. Briefly, healthy cells respiring via the electron transport chain will absorb and reduce CTC

The RedoxSensor™ Green reagent included in the BacLight™ RedoxSensor™ Green Vitality Kit is an indicator of bacterial reductase activity. This reductase activity is, in turn, a reliable marker for changes in electron transport chain function and for chan

Can be used to control the level of intracellular Ca2+BAPTA is more selective for Ca2+ than EDTA and EGTA, and its metal binding is also less sensitive to pHCalcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis a

Can be used to control the level of extracellular Ca2+BAPTA is more selective for Ca2+ than EDTA and EGTA, and its metal binding is also less sensitive to pHCalcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis a

BAPTA is more selective for Ca2+ than EDTA and EGTA, and its metal binding is also less sensitive to pHCalcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling

For measuring changes ± 1 pH unit around pH 7pKa of approximately 6.98pH measurements with BCECF are made by determining pH-dependent ratio of emission intensity (detected at 535nm) when dye is excited at approximately 490nm versus emission intensity whe

With pKa of approximately 6.98, cell-permeant, dual-excitation ratiometric pH indicator, AM is ideal for measuring changes in cytosolic pH of most cellsIntracellular pH measurements with BCECF are made by determining pH-dependent ratio of emission intens

BTC, AM is a cell-permeant coumarin benzothiazole-based Ca2+ indicator. It exhibits a shift in excitation maximum from about 480 nm to 400 nm upon binding Ca2+, which permits ratiometric measurements that are essentially independent of uneven dye loading

Calcein AM is a cell-permeant dye frequently used in cell biology research. It is derived from Calcein, a fluorescent dye that exhibits bright green fluorescence upon binding to intracellular free calcium ions. Calcein AM is a non-fluorescent compound th