ROS-Nitric Oxide Pathway Reagents and Kits

The CellROX Green Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX Green Reagent, as well as SYTOX Red Dead Cell Stain, N-acetyl cysteine (an an

Aminophenyl fluorescein (APF) and hydroxyphenyl fluorescein (HPF, H36004), two reactive oxygen species (ROS) indicators developed by T. Nagano, offer greater specificity and stability than dichlorodihydrofluorescein diacetate (H2DCFDA, D399).APF and HPF

BODIPY™ 581/591 C11 undecanoic acid (Lipid Peroxidation Sensor) is a sensitive fluorescent probe for lipid peroxidation assays that localizes to membranes in live cells. Oxidation of the polyunsaturated butadienyl portion of this fatty acid analog in liv

BODIPY 665/676 is a lipophilic probe used to detect lipid peroxidation. It exhibits a change in fluorescence after interaction with peroxyl radicals within cells. We recommend dissolving BODIPY 665/676 in high-quality anhydrous DMSO to attain a stock con

6-carboxy-2',7'-dichlorodihydrofluorescein diacetate is chemically reduced, acetylated form of fluorescein used as indicator for reactive oxygen species (ROS) in cells. This nonfluorescent molecule is readily converted to green-fluorescent form when acet

H2DFFDA is nonfluorescent molecule that readily converts to green-fluorescent form when acetate groups are removed by intracellular esterases and oxidation (by the activity of ROS) occurs within the cell. This fluorinated analog exhibits improved photost

Cell-permeant dye is weakly fluorescent while in reduced state and exhibits bright green photostable fluorescence upon oxidation by reactive oxygen species (ROS) and subsequent binding to DNA, with absorption/emission maxima of ∽485/520 nm. This reagent

The CellROX™ Orange Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Orange Reagent, as well as SYTOX™ Red Dead Cell Stain, N-acetyl cysteine (

CellROX™ Orange Reagent is a novel fluorogenic probe for measuring oxidative stress in live cells. This cell-permeant dye is non-fluorescent while in a reduced state and exhibits bright orange fluorescence upon oxidation by reactive oxygen species (ROS),

The CellROX Variety Pack contains one vial (50μL) each of CellROXGreen Reagent, CellROXOrange Reagent, and CellROXDeep Red Reagent. These reagents are novel fluorogenic probes for measuring cellular oxidative stress in live cells. These cell-permeant dye

Kit leverages copper-catalyzed click chemistry and linoleamide alkyne (LAA) reagent (alkyne-modified linoleic acid) to detect lipid peroxidation-derived protein modifications in fixed cells. Linoleic acid is most abundant polyunsaturated fatty acid found

CM-H2DCFDA is a chloromethyl derivative of H2DCFDA, useful as an indicator for reactive oxygen species (ROS) in cells. This indicator exhibits much better retention in live cells than H2DCFDA. CM-H2DCFDA passively diffuses into cells, where its acetate g

DAF-FM is essentially nonfluorescent until it reacts with NO to form fluorescent benzotriazole. DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and

It is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fl

6-carboxy-2',7'-dichlorodihydrofluorescein diacetate is nonfluorescent molecule is readily converted to green-fluorescent form when acetate groups are removed by intracellular esterases and oxidation (by the activity of ROS) occurs within cell. Upon clea