Cell Based Assays

An amphipathic polymer ideally suited to improve solubilization and stabilization of membrane proteins while maintaining structural integrity and activityDetergent-free substitute to extract and stabilize membrane proteins in aqueous solutionsSurfactant

Dimethyl sulfoxide, or DMSO, is a highly polar organic solvent that is miscible with water. Many compounds that are not directly soluble in water can be dissolved with DMSO to create a concentrated stock solution that can be further diluted to the desire

Protocols for crosslinking molecules with this reagent can be found in Bioconjugate Techniques, by G.T. Hermanson (B-7884).

Iodoacetamido-LC-Phosphonic Acid (6C-CysPAT) is a sulfhydryl-reactive alkylating reagent that contains a phosphonic acid group for enrichment of cysteine-containing peptides for mass spectrometry analysis. A sulfhydryl-reactive alkylating reagent that co

Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.PI binds to DNA by intercalating between the bases

The Thermo Scientific VitroEase Buffer Screening Kit provides pre-formulated buffers and detergents and a comprehensive screening strategy to identify optimal protein conditions for single particle cryo-EM sample analysis. The Thermo Scientific VitroEase

An easy, ready-to-use negative stain for electron microscopy (EM) analysisProvided as a stable, ready-to-use 2% stain solutionEasy to use with straight-forward staining protocolUniform, electron-dense stain for EMWets both grid films and specimens well a

An easy, ready-to-use negative stain for electron microscopy (EM) analysisProvided as a stable, ready-to-use 2% stain solutionEasy to use with straight-forward staining protocolExcellent spreading properties at a near-neutral pH of 8Uniform, electron-den

The VitroEase™ Cryo-EM Training Kit has been designed to provide the necessary components to get started with cryo-EM analysis of protein samplesSample preparation is a major bottleneck in cryo-electron microscopy (cryo-EM) single particle analysis (SPA)