3,3?-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
• 3,3'-Diaminobenzidine tetrahydrochloride hydrate is an organic compound used as a peroxidase substrate
Application
• 3,3'-Diaminobenzidine tetrahydrochloride hydrate is commonly used for immunoblotting and immunohistochemical staining techniques
• It has also been used for the histological stain to measure the peroxidase activity. The interaction between 3,3'-Diaminobenzidine tetrahydrochloride hydrate and peroxidase leads to the formation of a visible insoluble product. Therefore, it has been applied to measure glutamate decarboxylase activity in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces
• It is used as a chromogen for detecting the antigen-antibody complex
• This compound is also used in several laboratory applications, including electron and light microscope, and FISH (fluorescence in situ hybridization)