5-Bromo-3-indolyl-β-D-galactopyranoside is an α-galactosidase substrate which is converted to an insoluble indigo-blue chromophore darker than that released by X-GAL. It is ideal for Lac gene detection systems in immunoblotting, immunocytochemical, and histological applications. Also used as chromogenic substrate that is suitable for identification of lac-(+) bacterial colonies.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications5-Bromo-3-indolyl-β-D-galactopyranoside is an α-galactosidase substrate which is converted to an insoluble indigo-blue chromophore darker than that released by X-GAL. It is ideal for Lac gene detection systems in immunoblotting, immunocytochemical, and histological applications. Also used as chromogenic substrate that is suitable for identification of lac-(+) bacterial colonies.
SolubilitySoluble in dimethylformamide (100 mg/ml), dimethylformamide/water (1:1 v/v) (10 mg/ml - clear, practically colorless solution).
NotesStore in freezer (-20°C). Keep the container tightly closed. Store away from oxidizing agent.