CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. It is suitable for isolation of membrane proteins with the following features such as sufficient protein solubilization capability, no denaturing or inactivation of proteins, no interference with protein activities, no precipitation at 4°C, appropriate critical micelle concentrations (CMC) and micelle size, no absorption in the UV region, no toxicity and availability of detergent detection methods.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
• CHAPS (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate) is a zwitterionic non-denaturing detergent
• It is suitable for applications which require protection of native protein conformation
Applications
• CHAPS can be used for the solubilization and purification of membrane proteins
• It is also used in conjunction with nonionic detergents like Triton X-100
• It can be used as a non-denaturing zwitterionic detergent for the study of membrane chemistry