Protein Extraction and Purification Reagents and Kits

Thermo Scientific™ RIPA Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells. This RIPA buffer effectively lyses and extracts protein from cultured mammalian cells, including

An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturationAll B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal a

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin. The specially prepared nickel-NTA support consists of beaded agarose derivatized with th

Detergents and surfactants used to prepare protein and peptide samples can interfere with analysis by ELISA, isoelectric focusing, and mass spectrometry (MS). Removing detergents from peptide samples is especially challenging and critical for MS analysis

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin. The specially prepared nickel-NTA support consists of beaded agarose derivatized with th

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin. The specially prepared nickel-NTA support consists of beaded agarose derivatized with th

Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes. Isolation of mitochondria is typically a la

Thermo Scientific™ M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells. The complete cell lysis reagent is a nondenaturing detergent formulation that dissolves cell mem

Thermo Scientific™ Pierce Protein L Agarose is a versatile, high-performance affinity resin for antibody purification that is available as bottled agarose beads, pre-packed spin columns, 96-well filter plates and complete IgG purification kits. Pierce Pr

Thermo Scientific™ NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The NE-PER Kit is a nuclear protein extraction method that inv

Thermo Scientific™ Pierce IP Lysis Buffer is optimized for cell lysate yield, purity and compatibility with immunoprecipitation. The IP Lysis Buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS. This mod

Perform segregation and enrichment of proteins from five different cellular compartments with Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells. The kit enables the stepwise separation and extraction of cytoplasmic, membrane, nu

Thermo Scientific™ A549 (+hIGF-1) Control Lysate is a stimulated cell lysate that has been validated for the expression levels and phosphorylation of key targets in the AKT signaling pathway. Validated for the expression of AKT, PTEN, IRS1, IGF1-R, GSK3a

ActivX™ FP Probes feature a reactive fluorophosphonate group that specifically and covalently labels the active-site serine of enzymatically active serine hydrolases. These probes are available with a desthiobiotin (biotin analog) tag for selective enric

ActivX Desthiobiotin-ATP or ADP Probes are nucleotide derivatives that covalently modify the active site of enzymes at conserved lysine residues in the nucleotide binding site. The structure of these probes consists of a modified biotin (desthiobiotin) a