Cell Function Reagents and Kits - Pages 4

Can be used to control the level of extracellular Ca2+BAPTA is more selective for Ca2+ than EDTA and EGTA, and its metal binding is also less sensitive to pHCalcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis a

BAPTA is more selective for Ca2+ than EDTA and EGTA, and its metal binding is also less sensitive to pHCalcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling

For measuring changes ± 1 pH unit around pH 7pKa of approximately 6.98pH measurements with BCECF are made by determining pH-dependent ratio of emission intensity (detected at 535nm) when dye is excited at approximately 490nm versus emission intensity whe

With pKa of approximately 6.98, cell-permeant, dual-excitation ratiometric pH indicator, AM is ideal for measuring changes in cytosolic pH of most cellsIntracellular pH measurements with BCECF are made by determining pH-dependent ratio of emission intens

BTC, AM is a cell-permeant coumarin benzothiazole-based Ca2+ indicator. It exhibits a shift in excitation maximum from about 480 nm to 400 nm upon binding Ca2+, which permits ratiometric measurements that are essentially independent of uneven dye loading

Calcein AM is a cell-permeant dye frequently used in cell biology research. It is derived from Calcein, a fluorescent dye that exhibits bright green fluorescence upon binding to intracellular free calcium ions. Calcein AM is a non-fluorescent compound th

Kd of indicator can be calculated from plot generated by scanning excitation or emission of indicator in presence of eleven different Ca2+ concentrationsCalcium Calibration Kit #1 contains 50mL of 10mm K2EGTA and 50mL of 10mm CaEGTABoth solutions contain

Uses in many calcium signaling investigations, including measuring intracellular Ca2+, following Ca2+ influx and release, and multiphoton excitation imaging of Ca2+ in living tissuesCells may be physically loaded with the cell-impermeant salt forms of th

Uses in many calcium signaling investigations, including measuring intracellular Ca2+, following Ca2+ influx and release, and multiphoton excitation imaging of Ca2+ in living tissuesCells may be physically loaded with the cell-impermeant salt forms of th

They have uses in many calcium signaling investigations, including measuring intracellular Ca2+, following Ca2+ influx and release, and multiphoton excitation imaging of Ca2+ in living tissuesCells may be loaded with the AM ester forms of these calcium i

Exhibits an increase in fluorescence emission intensity upon binding Ca2+ with little shift in wavelengthEssentially nonfluorescent in absence of Ca2+ and low-affinity indicator has dissociation constant for Ca2+ in absence of Mg2+ of approximately 14μMC

These indicators have uses in many calcium signaling investigations, including measuring intracellular Ca2+, following Ca2+ influx and release, and multiphoton excitation imaging of Ca2+ in living tissuesCells may be loaded with the AM ester forms of the

Carboxy-DCFDA can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pKa of 4.8, this

This fluorescein-based probe contains two galactoside moieties, making it a target for β-galactosidase. Activation of β-galactosidase is commonly used as a biomarker for senescent cells. This hydrolase enzyme resides in lysosomes and converts β-galactosi

The kit contains an optimized buffer and a fluorescein-based reagent that contains two galactoside moieties, making it a specific target for β-galactosidase. Activation of β-galactosidase is commonly used as a biomarker for senescent cells. This hydrolas