PBS with EDTA is also used to disengage attached and clumped cells. PBS-EDTA is used as a buffer in procedures generating dendritic cells from monocytes. PBS+EDTA to get better cell sorting. PBS/EDTA may also be used directly without addition of trypsine for very gentle cell dissociation. PBS can be used to take a reference spectrum when measuring the protein adsorption in ellipsometry. PBS can be used as a diluent in methods to dry biomolecules.
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