Champion™ pET100 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli

Based on T7 expression vectors originally developed by Studier and colleagues, high-level expression is achieved because T7 RNA polymerase is more processive than native E. coliRNA polymerase and is dedicated to transcription of gene of interestProtein p