Keep as concentrated solution. Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%). Positive Control: Neuro2A, NIH-3T3, Raw264.7, C2C12, NIH-3T3 (starvation for 16 hr), NIH-3T3 (starvation for 16 hr and 100 ng/ml PDGF treatment for 5 min), EGF