0.5 μg/lane, 8 cm length gel, 1X TBE, 5 V/cm, 1.5 h. Bands visible: 501, 489, 404, 331, 242, 190, 147, 111, 110, 67, 34 and 34 bp.pUC19 DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme, then purified and dissolved i