0.5 μg/lane, 8cm length gel, 1X TBE, 5V/cm, 1.5h. Bands visible: 1353, 1078, 872, 603, 310 281, 271, 234, 194, 118, and 118 bp.phiX174 DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme, then purified and dissolved in