Modifying Enzymes and Buffers

Thermo Scientific Exonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact. It does not cleave DNA strands with terminal 3'

RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions. Does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and HPerforms under a wide ra

RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues.It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an ad

Gateway™ LR Clonase™ II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by attL sites) and a destination vector (containing attR sites) to generate an expression cloneIt contains enzymes and b

Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also

Catalyzes the in vitro recombination of PCR products or subcloning DNA segments from clones (containing attB sites) and a donor vector (containing attP sites) to generate entry clonesContains enzymes and buffer in a single mix to enable convenient ten-mi

Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also

Cleaves double-stranded DNA nonspecifically to leave 5' phosphorylated oligodeoxynucleotides with an increased affinity for DNA-binding and remains active in presence of salt.Up to 50X more activity and 350% greater catalytic efficiencyEfficiently degrad

The 5X Kinase Buffer A is a concentrated general kinase assay buffer that can be used with a variety of assay technologies such as, LanthaScreen™, Adapta™, and Z’-LYTE™ assays.Ready to use and validated with LanthaScreen™, Adapta™, and Z’LYTE

An improved version of Tobacco Etch Virus (TEV) AcTEV™ Protease is highly site-specific, highly active and significantly more stable than native TEV protease, resulting in enhanced long-term activity.Highly specific cleavage activityEnhanced enzyme stabi

Agarase specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides. Agarase allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose. The recovered nucleic acids can be directly used for amp

DNase I functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with 5'-phosphate and 3'-hydroxyl group. RNase-free DNase I is recommended to degrade DNA in presence of RNA, in absence of RNase is critical to maintain integr

For large batch sizes, use of RNase is still only effective technique applicable to remove host RNA. Other methods such as selective precipitation, gel filtration, chromatography, and endogenous E. coli RNase digestion have only been effective for small-

Ambion RNase A is endonuclease that specifically cleaves 3' of U and C residues. Affinity Purified RNase A is intended for critical applications when absence of DNase and other nonspecific nuclease activities is essential.Supplied in one tube containing

Ribonuclease H (RNase H) is isolated from an E. coli strain that over-expresses the gene. RNase H specifically degrades RNA in RNA:DNA hybrids to produce 3'-hydroxyl and 5'-phosphate terminated products.Will not degrade DNA or unhybridized RNAIntegral pa