Crosslinking, Labeling and Protein Modification - Pages 12

The Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy or high-content imaging. In this assay O-propargyl-puromycin (OPP) is efficiently incorporated into newly translated proteins in complete methionine-containing media and fluorescently labeled with a bright, photostable Alexa Fluor™ dye in a fast, highly-specific, and mild click reaction. Features of the kit include: No media change required—works in complete, methionine-containing media, no need to remove cell media Multiplex-enabled—Click-iT™ Plus technology retains signal from GFP and binding of fluorescent-conjugated phalloidins Non-radioactive—an alternative to the traditional 35S-methionine methods Works in vivo—published results demonstrate use in vivo for determination of protein translation The kit contains O-propargyl-puromycin (OPP), which is an alkyne analog of puromycin (also available

The Click-iT™ Plus OPP Alexa Fluor™ 594 Protein Synthesis Assay Kit provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy or high-content imaging. In this assay O-propargyl-puromycin (

The Click-iT™ Plus OPP Alexa Fluor™ 647 Protein Synthesis Assay Kit provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy or high-content imaging. In this assay O-propargyl-puromycin (

The Click-iT Protein Enrichment Kit for the efficient capture of click chemistry based azide-modified proteins on a resin of alkyne agarose. Superior to biotin or lectin based enrichment approaches. Ideal for proteomics, biomarker discovery, post-transla

The Click-iT Protein Buffer Kit provides researchers who have proteins labeled with an azide or alkyne and the corresponding click detection reagent with the means to click these two moieties together. Each kit includes sufficient material to perform 25

Achieve efficient labeling of azides with Click-It sDIBO Alkynes. Our Click-IT sDIBO alkynes enable the labeling of azido labeled targets without the need for copper catalysis and are available with a variety of Alexa Fluor or biotin labels, as well as a

The Click-iT Tetramethylrhodamine (TAMRA) Glycoprotein Detection Kit provides the second part of the simple and robust two-step technique to identify and characterize glycoproteins by one- or two-dimensional gel electrophoresis. In step two, after the in

Conjugation of the succinimidyl ester of the water-soluble, CMNB-caged carboxyfluorescein, SE to a primary amine on a biomolecule of interest produces an essentially nonfluorescent probe that yields a green-fluorescent fluorescein-labeled product only af

Thermo Fisher Scientific offers a broad selection of Invitrogen concanavalin A (Con A) conjugates, including Alexa Fluor™ and Alexa Fluor™ Plus conjugates. Concanavalin A (Con A) has been used in a variety of applications, including immunofluorescence (I

The thiol-reactive coumarin, CPM is very weakly fluorescent until reacted with thiols producing a conjugate with excitation/emission maxima of ∼384/470 nm.

Thermo Scientific™ Pierce CT(PEG)12, or carboxy-PEG12-thiol, is a carboxyl- and sulfhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles

The amine-reactive quencher, dabcyl succinimidyl ester has a broad and intense visible absorption but no fluorescence making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.

The primary aliphatic amine of dansyl ethylenediamine can be reversibly coupled to aldehydes and ketones to form a Schiff base - which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3).

DIFMU can be used as a reference standard for ether, ester and phosphate substrates derived from this blue-fluorescent dye.

Disufide crosslinks of cystines in proteins can be reduced to cysteine residues by dithiothreitol (DTT).