Crosslinking, Labeling and Protein Modification - Pages 13

Thermo Scientific™ Pierce DMP is dimethyl pimelimidate, a membrane-permeable crosslinker that contains an amine-reactive imidoester group at each end of a 7-atom spacer arm. Highlights:Water-solubleRapid reaction with amines at alkaline pH values (pH 8-1

Thermo Scientific™ Pierce DMS is dimethyl suberimidate, a membrane-permeable crosslinker that contains an amine-reactive imidoester group at each end of an 8-atom spacer arm. Highlights:Water-solubleRapid reaction with amines at alkaline pH values (pH 8-

The amine-reactive DNP-X succinimidyl ester can be used bioconjugates that can be detected with anti-dinitrophenyl (anti-DNP) antibodies. This reactive hapten contains an additional seven-atom aminohexanoyl spacer ('X') between the fluorophore and the su

Dolichos Biflorus (Horse Gram) Agglutinin (DBA), fluorescein (FITC), is a bright-green DBA-fluorophor conjugate. DBA has been used to establish secretor status in blood-group-A individuals by hemagglutination inhibition techniques and for blood typing.Th

Thermo Scientific™ Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm. Highlights:Chemical crosslinkin

Thermo Scientific™ Pierce DST, also called disuccinimidyl tartrate, is a water-soluble crosslinker that contains amine-reactive NHS ester ends around a 4-atom spacer arm, whose central diol can be cleaved by periodate oxidation. Highlights:Cleavable by S

Thermo Scientific™ Pierce DTBP is dimethyl 3,3'-dithiobispropionimidate, a crosslinker that contains amine-reactive imidoester groups around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents. Highlights:Water-solubleC

Thermo Scientific™ Pierce DTME is a mid-length, maleimide crosslinker for reversible covalent conjugation between sulfhydryl groups (e.g., protein or peptide cysteines) by reduction of the disulfide bond in the center of the spacer arm. Highlights:Cleava

Thermo Scientific™ Pierce DTNB (Ellman's Reagent) reacts with sulfhydryl groups to yield a colored product, which provides a reliable method for measure free reduced cysteines and other free sulfhydryls in solution. A solution of this compound produces a

DTNB or Elman's reagent can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readily forms a mixed disulfide with thiols, liberating the chromophore 5-merapto-2-nitrobenzoic acid (absorption maximum 410 nm). Only

Thermo Scientific™ Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm and whose central disulfide bond can be cleaved with reducing agents. Highlights:Water-solubleCleavable by redu

DTT is an essential component for kinase assays using LanthaScreen™ Technology. Invitrogen's DTT is 1M in water. It is tested for background fluorescence, making it optimal for use in high-throughput screening applications.

Thermo Scientific™ Amine-Reactive DyLight Dyes are NHS ester-activated DyLight Fluors for fluorescent labeling of antibodies and other proteins to be used as molecular probes for cellular imaging and other fluorescence detection methods. The DyLight Dyes

Thermo Scientific™ Sulfhydryl-Reactive DyLight Dyes are maleimide-activated DyLight Fluors for fluorescent labeling of antibodies and other proteins to be used as probes for cellular imaging and other fluorescence detection methods. The Thermo Scientific

Thermo Scientific™ Amine-Reactive DyLight Dyes are NHS ester-activated DyLight Fluors for fluorescent labeling of antibodies and other proteins to be used as molecular probes for cellular imaging and other fluorescence detection methods. The DyLight Dyes