Crosslinking, Labeling and Protein Modification - Pages 2

5(6)-TAMRA contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. This bright, orange-fluorescent dye produces conjugates with absorption/emission maxima of ∼555/580 nm.

The amine-reactive 6-(tetramethylrhodamine-5-(and-6)-carboxamido)hexanoic acid, succinimidyl ester (5(6)-TAMRA-X, SE) can be used to can be used to create bright red-orange fluorescent bioconjugates with excitation/emission maxima ∼555/580 nm.

The amine-reactive form of 5-CR 6G is used for oligonucleotide labeling and automated DNA sequencing applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine™ rhodamine B conjugates, but somewhat shorter-wavelengt

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The carboxylic acid of 5-ROX is used for oligonucleotide labeling and automated DNA sequencing applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine™ rhodamine B conjugates, but somewhat shorter-wavelength spec

The isomeric 5-ROX, SE is an amine-reactive form of carboxy-X-rhodamine and is widely used for oligonucleotide labeling and automated DNA sequencing applications.

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Tetramethylrhodamine (TMR, TRITC) has been a widely used fluorophore for preparing bioconjugates, especially fluorescent antibody and avidin derivatives used in immunochemistry. Under the name TAMRA, the carboxylic acid of 5-TAMRA has also achieved promi

The amine-reactive 5-TAMRA, SE and its conjugates yield bright, pH-insensitive orange-red fluorescence (approximate excitation/emission maxima ∼546/579) with good photostability.

The single isomer, 6-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In additio

This single isomer preparation of the amine-reactive dye 6-JOE, SE has fluorescence characteristics that are red-shifted as compared to fluorescein (excitation/emission is 522/550 nm) and is one of the traditional fluorophores used in automated DNA seque

The excitation and emission spectra of carboxyrhodamine 6G (CR 6G) fall between those of fluorescein and tetramethylrhodamine. With a peak absorption at 525 nm, conjugates of carboxyrhodamine 6G are an excellent match to the 514 nm spectral line of the a

The isomeric 6-ROX, SE is an amine-reactive form of carboxy-X-rhodamine and is widely used for oligonucleotide labeling and automated DNA sequencingapplications.