Modifying Enzymes and Buffers - Pages 2

RNase I degrades all RNA dinucleotide bonds leaving a 5' hydroxyl and 2', 3' cyclic monophosphate. RNase I will degrade any RNA to mixture of mononucleotides, dinucleotides, and trinucleotides and does not degrade DNA, although it will bind to DNA. It ha

Escherichia coli Ribonuclease III (RNase III; EC 3.1.24) is specific endoribonuclease. In E. coli, RNase III cleaves ribosomal RNA (rRNA) precursors during maturation of rRNA. Enzyme cleaves dsRNA into 12–15 bp dsRNA fragments with 2 to 3 nucleotide 3' o

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. The Invitrogen Anza Restriction Enzyme Cloning System utilizes a single buffer system for complete digestions and incorporates Anza cloning enzy

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. The Invitrogen Anza Restriction Enzyme Cloning System utilizes a single buffer system for complete digestions and incorporates Anza cloning enzy

The Invitrogen™ Anza™ DNA End Repair Kit is used to convert DNA with cohesive ends to blunt-ended 5’-phosphorylated DNA for use in blunt-end ligation reactions. The included pre-mixed Anza™ DNA End Repair Mix contains T4 DNA polymerase, Klenow Frag

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. The Invitrogen Anza Restriction Enzyme Cloning System utilizes a single buffer system for complete digestions and incorporates Anza cloning enzy

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. The Invitrogen Anza Restriction Enzyme Cloning System utilizes a single buffer system for complete digestions and incorporates Anza cloning enzy

BAP is active at 65°C for at least 1 hr. and is inactivated by phenol extractionSource: Purified from E. coli C90Performance and Quality Testing: Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency determine

Bovine Serum Albumin is suitable for use as enzyme stabilizer during purification or for dilution of restriction endonucleases and nucleic acid modifying enzymes. BSA is also commonly used in DNA and protein labeling experiments as a blocking agent to mi

Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance an

Concentration: 20 units/μLSource: Purified from calf intestinal mucosaPerformance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assayUnit Definition

Concentration: 20 units/μLSource: Purified from calf intestinal mucosaPerformance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assayUnit Definition

These Control Vesicles are insect-derived purified plasma membranes with no inserted transport proteins for use as a negative control in vesicular transport assays. Our Control Vesicles control for nonspecific absorption and transport of test substrates

CorrectASE™ enzyme removes mismatches caused by oligonuceotide synthesis errors, leading to a 3–10 fold reduction in mutations in your synthetic genes or fragments.By introducing CorrectASE™ enzyme into your do-it-yourself gene syntheis workflow, you can

The enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5'-CpG-3' context.M.SssI is specifically formulated for fast reaction times without compromising the