Flow Cytometry Compensation, Counting and Calibration - Pages 2

Molecular Probes™ Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can b

Molecular Probes™ Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell sorting system, including laser source, optics, and stream flow. The beads are optimized for checking cell sorter settings such as drop delay and efficiency (cell loss during sorting). View information for all flow cytometry calibration and size reference beads. Cell Sorting Set-up Beads are available for use with UV, blue, green/yellow, and red lasers.These 6.0μm beads for UV lasers are fluorescent-dye infused microspheres that are excitable between 350–370 nm with emission in the range of 400–470 nm. Cell Sorting Set-Up Beads provide: Consistency in production, creating ideal reference standards Confidence through reliable, optimal instrument performance Compatibility—use with any inst

CountBright™ absolute counting beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range of excitation and emission wavelengths (UV to 635 nm excitation and 385-800 nm emission). CountBright™ absolute counting beads are mixed with the cell sample and assayed via flow cytometry. By comparing the ratio of bead events to cell events, absolute numbers of cells in the sample can be calculated. Because CountBright™ beads are mixed in the test sample, absolute cell counts using this single-platform method are more accurate and less complicated than cell concentration determined using multiple-platform testing. CountBright™ absolute counting beads can be used with any sample type, including no-wash/lysed whole blood. View information about all flow cytometry cell counting beads.

Help to determine the count.The concentration of cells in the original sample.Beads are on-scale with most cell types, including human whole lysed blood, for ease of use>95% singlets for improved accuracyExcited by UV-IR lasers for an expanded emission r

CountBright Plus Ready Tubes offer the same advantages as CountBright Plus Absolute Counting Beads, but in ready-to-use tubes. This not only improves the accuracy and repeatability of cell counting, but also allows for convenient room temperature storage

Phenotyping Kit leverages high-quality eBioscience™ antibodies and isotype controls and includes a ready-to-use protocol for experimental setup, gating strategy, and analysis. Benefits include:Proven performance―antibodies and supporting reagents designe

Essential Human T-Cell Phenotyping Panel everages high quality eBioscience™ antibodies and a ready-to-use protocol for experimental setup, gating strategy, and analysis. Benefits include:Proven performance―antibodies designed for flow cytometryHighly ref

Phenotyping Kit leverages high quality eBioscience™ antibodies, isotype controls, cell stimulation reagents (induces cytokine production), and fixation/permeabilization reagents (for intracellular markers IfN-g and IL-17) and includes a ready-to-use prot

Phenotyping Panel with Staining and Stimulation Reagents leverages high quality eBioscience™ antibodies, cell stimulation reagents (induces cytokine production), and fixation/permeabilization reagents (for intracellular markers IfN-g and IL-17) and inclu

Phenotyping Kit leverages high-quality eBioscience™ antibodies, isotype controls, and fixation/permeabilization reagents (for the intracellular marker FOXP3) and includes a ready-to-use protocol for experimental setup, gating strategy, and analysis. Bene

Phenotyping Panel and Staining Buffers leverages high quality eBioscience™ antibodies and fixation/permeabilization reagents (for the intracellular marker FOXP3) and includes a ready-to-use protocol for experimental set-up, gating strategy, and analysis.

Kit contains all the buffers, blockers, and beads needed to complete a standard flow cytometry experiment for staining cell surface targets on human cell suspensionsWhen combined with experiment-specific antibodies, the reagents within this kit have been

Kit contains all of the normal buffers, blockers, and beads to complete a standard flow cytometry experiment for staining intracellular cytoplasmic and cytokine targets in human cell suspensionsWhen combined with experiment-specific antibodies, the reage

Kit contains all of the normal buffers, blockers, and beads to complete a standard flow cytometry experiment for staining intracellular nuclear and transcription factor targets in human cell suspensionsWhen combined with experiment-specific antibodies, t

Kit contains all of the normal buffers, blockers, and beads to complete a standard flow cytometry experiment for staining cell surface targets on human lysed whole bloodWhen combined with experiment-specific antibodies, the reagents within this kit have