Crosslinking, Labeling and Protein Modification - Pages 25

Pacific Blue™ C5-maleimide is an excellent reagent for thiol-selective modification, quantitation and analysis. In this reaction, the thiol is added across the double bond of the maleimide to yield a thioether. This compound does not react with methionin

The amine-reactive Pacific Blue™ succinimidyl ester can be used to can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ∼410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser.View all P

Pacific Orange and Pacific Blue antibody labeling kits enable attachment of a violet light-excitable fluorescent label on antibodies (up to 100 μg per reaction). Each kit contains enough dye for five reactions. A primary antibody directly labeled with a

Pacific Orange™ succinimidyl ester is a violet-excitable (405 nm) dye with emission maximum at ∼551 nm. This allows it to be used with other violet-excitable fluorophores such as Pacific Blue™ dye for multiparameter analysis using a single excitation sou

The fluorescent diazomethyl derivative PDAM (1-pyrenylyldiazomethane) has been commonly used to derivatize biomolecules. Diazoalkane react with the addition of catalysis and may be used for direct carboxylic acid modification of proteins and synthetic po

Thermo Scientific™ Pierce PDPH is a short, pyridyldithiol-and-hydrazide crosslinker for reversibly conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates). Highlights:Pyridyl

Thermo Scientific™ Pierce PEG12-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a 12-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond. SPDP rea

Thermo Scientific™ Pierce PEG4-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a 4-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond. SPDP reage

Elevate biopharmaceutical research with pHrodo Antibody Labeling kits, designed to characterize antibody internalization, a critical step during antibody-drug conjugate research and development. Kits provide a simple, detailed protocol and advanced purif

Perform cell internalization studies with low signal-to-background and high specificity using the pHrodo pH sensor dyes, including pHrodo Red, Green, and Deep Red dyes. These dyes can be conjugated to biomolecules such as antibodies, and become highly fl

The thiol-reactive pH-sensitive pHrodo™ Green Maleimide dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo™ Green dramatically increases fluorescence as the pH of its surroundings become more acidic.Use pH-sen

The amine-reactive pH-sensitive pHrodo™ Green STP ester dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo™ Green dramatically increases fluorescence as the pH of its surroundings become more acidic.Use pH-sen

The thiol-reactive pH-sensitive pHrodo™ Red Maleimide dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo™ Red dramatically increases fluorescence as the pH of its surroundings become more acidic.Use pH-sensiti

Thermo Scientific™ Pierce 2-Mercaptoethanol (2-ME), also called beta-mercaptoethanol (b-ME), is a mild reducing agent for cleaving protein disulfide bonds. Highlights:A mild reducing agent for cleaving disulfide bonds to thiolsAlso known as β-Mercaptoeth

Thermo Scientific Pierce Iodoacetamide or Chloroacetamide are provided in a no-weigh format, where small amounts of reagent are pre-weighed and packaged for single use. This single-use format prevents the contamination and loss of reagent reactivity over