Crosslinking, Labeling and Protein Modification - Pages 26

The Pierce Antibody Biotinylation Kit reagents have been optimized and validated to biotin label antibodies for IP and co-IP reactions. Determining the optimal number of biotins to attach to the target molecule is one of the major challenges of biotinyla

Thermo Scientific™ Pierce Biotin Quantitation Kit includes aliquots of HABA reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins. Determine the molar ratio of

Features of the Cell Surface Protein Biotinylation and Isolation Kit include:• Improved performance—kit and protocol have been re-designed to improve enrichment and extraction of biotinylated plasma membrane proteins while minimizing intracellular protei

Thermo Scientific™ Pierce Citraconic Anhydride reacts with lysines and other primary amines to stably cap them with a carboxyl group at pH 7, but whose linkage can be completely reversed (hydrolyzed) using acidic conditions (pH 3 to 4). Highlights:Reacts

Thermo Scientific™ Pierce Conjugate Purification Kit is a unique affinity chromatography system for purifying horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated antibodies from unreacted enzyme after labeling. The metal chelate ligand o

Thermo Scientific™ Pierce Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes. The kit provides Sulfo-SMCC crossli

Thermo Scientific™ Pierce Cysteine-HCl is purified, crystalline cysteine hydrochloride salt (MW 175.6) that is useful as a sulfhydryl standard in assay with Ellman's Reagent, or as an additive in protein refolding experiments. Thermo Scientific™ Pie

Thermo Scientific™ Pierce DSG is disuccinimidyl glutarate, a simple homobifunctional crosslinker based on the amine-reactive N-hydroxysuccinimide (NHS) ester group. Highlights:Can increase crosslinking efficiency compared to DSS in some applicationsReact

Thermo Scientific™ Pierce DSP Crosslinker (Lomant's Reagent) is an amine-reactive crosslinker with NHS-ester reactive ends and a cleavable disulfide bond in its spacer arm. Highlights:Cleavable by reducing agentsMembrane-permeableAcylate in aqueous or or

Dithiothreitol is a popular reducing agent for many protein research applications, including reducing polyacrylamide electrophoresis (SDS-PAGE). Dithiothreitol is a popular reducing agent for many protein research applications, including reducing polyacr

Thermo Scientific™ Pierce DTT (Dithiothreitol) is offered as 7.7 mg x 48-tube microplates for convenient preparation of 100 μL of 0.5 M stocks for addition to protein samples for effective reduction of disulfide bonds. Thermo Scientific Pierce DTT (Dithi

Thermo Scientific™ Pierce Dye Removal Columns effectively bind to unconjugated fluorescent dyes molecules from protein solutions to rapidly purify fluorescent conjugated antibodies and other proteins after labeling reactions. Highlights:Fast - removal fr

Thermo Scientific™ Pierce EDC is a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation. EDC is the abbreviation for 1-E

Thermo Scientific™ FITC and Fluorescein Labeling Reagents and Kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes. Fluorescein dyes a