Nucleic Acid Labeling and Detection - Pages 3

Nucleic Acid Detection Blocking Buffer is intended for use with the Thermo Scientific Chemiluminescent Nucleic Acid Detection Module Kit, of which it is also a component. Non-specific binding of probe to membrane can be minimized by incubating the membra

Thermo Scientific™ Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods. TdT exhibits a substrate pre

Thermo Scientific™ Pierce RNA 3' End Biotinylation Kit is optimized for labeling the 3-prime terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions. The complete kit contain

Make DNA quantitation easy and accurate in a ready-to-go 1X formulation with the Quant-iT 1X dsDNA HS (High-Sensitivity) and Quant-iT 1X dsDNA BR (Broad Range) Assay kits. Both kits provide a ready-to-use working solution. Simply load your sample, add th

The Quant-iT™ microRNA Assay Kit allows easy and accurate quantification of microRNA (miRNA), even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for microRNA over rRNA or large mRNA (>1000 bp). Although the reagent is not exclusively selective for miRNA, we have been able to reproducibly quantify miRNA in pure samples at levels as low as 0.5 ng following the supplied protocol, even in the presence of mRNA.Features of Quant-iT™ microRNA Assay Kit: Accurately detects as little as 0.5 ng miRNA in a microplate wellCore dynamic range of 5–500 ng/mLAccurate results for sample concentrations from 50 ng/mL to 100μg/mL The Quant-iT™ microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent 1:200, load 200μL into the wells of a microplate, add 1–20μL of sample, mix, then read the fluorescence. The assay is performed at room temperatu

Quantify ssDNA with ease using the Quant-iT OliGreen ssDNA Assay kit and reagent. This assay kit and reagent enable sensitive quantitation of single-stranded DNA without the need for a highly concentrated sample. As little as 100 pg/mL can be quantitated

The Quant-iT PicoGreen ReadyPlate is a preloaded 96-well plate that offers a rapid, ultra-sensitive, and selective method for double-stranded DNA quantification. The plate is prefilled with buffer and PicoGreen reagent, which exhibits bright green fluore

Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers. Rapidly detect as lit

The Quant-iT RiboGreen ReadyPlate is a preloaded 96-well plate that offers a rapid, simplified, and highly sensitive method for RNA quantification. The ReadyPlate is prefilled with buffer and RiboGreen reagent, which exhibits bright green fluorescence up

Enable easy and accurate RNA quantitation with the Quant-iT RNA Assay Kit, which is also available in broad range (BR) and extended range (XR) configurations, as well as a stand-alone high sensitivity (HS) reagent. These Quant-iT RNA quantification kits

Achieve precise quantitation of dsDNA or RNA samples with stand-alone Qubit nucleic acid standards. The Qubit standards can be used in conjunction with different Qubit or Quant-iT nucleic acid quantitation assay kits and offer stand-alone versions of the

Quickly and easily determine RNA degradation with the Qubit RNA IQ Assay Kit and Qubit RNA IQ Standards. RNA integrity and quality (IQ) information is assessed using the assay kit's two unique dyes: one dye that binds to large and/or highly structured RN

ReadyProbes In Situ Hybridization (ISH) Blocking Solution minimizes non-specific background staining in chromogenic in situ hybridization (CISH) or fluorescence in situ hybridization (FISH) proceduresIt is provided as a 5X solution that is diluted to 1X

For the labeling of siRNA with Cy™3. Includes sufficient reagents for labeling 65μg of siRNA. Invitrogen™ Labeled siRNA can be used to analyze siRNA subcellular localization, stability, and transfection efficiency. In addition, fluorescently labeled siRN

The kit includes Invitrogen's proprietary RNase H reduced activity SuperScript™ II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix– protocol. Designed to consistently produce higher yiel