Modifying Enzymes and Buffers - Pages 5

Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance an

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.Proteinase K Remains Active:• Over a wide pH range (optimal activity between 6.5 and 9.5)• Under denaturing conditions (e.g

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a te

Thermo Scientific Pyrophosphatase, Inorganic catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates (see Figure 1 in Supporting data). The enzyme requires a divalent metal cation with Mg2+ conferring the highest activity. Highlights

Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations. Complete digestion of DNA and safe removal of DNase I from the digestion reaction is ensured without RNA damaging steps, such as heati

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:Over a wide pH range (optimal activity between 6.5 and 9.5)Under denaturing conditions (e.g., in the presence of SDS o

Source: Purified from E. coli expressing the E. coli RNase H gene on a plasmidPerformance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonucleaseUnit definition: One unit hydrolyzes 1nmol of RNA in 3H-labeled

RNase Cocktail™ is mixture of two highly purified ribonucleases, RNase A (500U/mL) and RNase T1 (20,000U/mL) and is free of DNase and nicking activities. Digestion of RNA with RNase A alone leaves fragments of RNA which are large enough to be visible on

Thermo Scientific™ Fermentas Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids. Does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA Quality Control:The absence of endo-, exodeo

Ribonuclease I (RNase I), an endoribonuclease, preferentially hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates via nucleoside 2', 3'-cyclic monophosphate intermediates.The enzyme does not require any metal ions for activity. HighlightsStabl

Thermo Scientific Rnase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues (see Figure 1 in Supporting Data). It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleot

Hydrolyzes single-stranded regions in duplex DNA such as loops and gaps.S1 Nuclease is stable at 65°C.Applications: Nuclease mapping techniques, Removal of single-stranded regions from double-stranded DNA, Exo III-ordered sequencingSource: Isolated from

S1 Nuclease degrades single-stranded nucleic acids, releasing 5'-phosphoryl mono- or oligonucleotides. It is five times more active on DNA than on RNA . S1 Nuclease also cleaves dsDNA at the single-stranded region caused by a nick, gap, mismatch or loop.

T4 β-glucosyltransferase (T4 BGT) transfers the glucose moiety of uridine diphosphoglucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA generating β-glucosyl-5 hydroxymethylcytosine.Thermo Scientific T4 BGT is spec

T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3'hydroxyl and 5'phosphate termini. Single-stranded nucleic acids are not substrates for this enzyme.Optimizes ligation in 5 minutesSou