Modifying Enzymes and Buffers - Pages 6

The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme.Unit Definition: One unit catalyzes the exchange of 1nmol 32P-labeled pyrophosphate into ATP in 20 m

As supplied with T4 DNA LigaseFormulation: 250mM Tris-HCl (pH 7.6), 50mM MgCl2, 5mM ATP, 5mM DTT, 25% (w/v) polyethylene glycol-8000Cloning (blunt-end or cohesive-end ligation), Adding linkers or adapters to blunt-ended DNA, ChIP-on-Chip, Chromatin Biolo

Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also

Thermo Scientific™ T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3'-monophosphates (forward reaction). Active in re

T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA.The minimal substrate is a nucleoside 3',5'-biphosphate in

Thermo Scientific™ Fermentas Terminal Deoxynucleotidyl Transferase (TdT), a template-independent DNA polymerase, catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH of oligodeoxyribonucleotides and single-stranded and double-stranded D

Formulation: 500mM potassium cacodylate (pH 7.2), 10mM CoCl2 , 1mM DTTHomopolymer tailing of vector and insert for cloning; Labeling oligonucleotides with biotin, 32P- or 35S-label (3); Apoptosis (TUNEL); Cloning; Restriction Enzyme Cloning

Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is DNA polymerase that catalyzes addition of deoxynucleotides to 3fi hydroxyl terminus of DNA.Source: Purified from baculovirus clone of calf thymus TdTPerformance and Quality Testing: Endonucleas

Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines. These products offer full workflow solutions from template preparation to RNA synthesis and are r

Thermo Scientific™ Thermus thermophilus DNA Ligase is a thermostable NAD-dependent DNA ligase that catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. Thermus thermophilus DNA Ligase is not acti

Topoisomerase I is active in the presence of EDTASource: Purified from calf thymusPerformance and Quality Testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease, and phosphatase assays; conversion of super-coiled DNA to relaxed DNAUnit Definition

Invitrogen TrueCut Cas9 Protein v2 is a next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency. Features include:Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, wi

A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiencyNear complete elimination of off-target events in all tested cell lines including standard, immune, p

Invitrogen TrueCut HiFi Cas9 Protein is a high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. Features include:Near complete elimination of off-target ev

Concentration: 20μg/μL in RNase-free waterPerformance and quality testing: No DNase or RNase activity detectedChIP-on-Chip, Chromatin Biology, DNA & RNA Purification & Analysis, DNA Extraction, General gDNA Purification Reagents & Accessories, Genomic DN