DNA Vectors - Pages 6

Pichia pastoris Expression Systems, Protein Expression, Proteins, Expression, Isolation and Analysis, Yeast Expression

The pIZT/V5-His vector features the OpIE2 promoter for constitutive expressionThe Zeocin resistance gene for rapid selection of stably transfected cell linesC-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Anti-V5 Antibody and

The S. cerevisiae strain INVSc1 is a fast-growing strain ideal for expression. INVSc1 has the following genotype:INVSc1: MATa his3D1 leu2 trp1-289 ura3-52 MAT his3D1 leu2 trp1-289 ura3-52

Tag is small (six amino acid) sequence that binds fluorescent substrate, allowing to visually detect protein localization in mammalian cellsOffers accurate in vitro and in vivo protein detectionCMV promoter for high-level constitutive expressiontR sites

The proprietary formulation enables E. coligrowth to reach culture densities 3 to 10-fold higher than those achieved with traditional LB + IPTG methodsAchieve 3 to 10 times higher protein yields with much less hands-on time Eliminate time-consuming O.D.

Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Cloning, Constitutive Expression, Enzyme and Protein Activity Assays, Gateway Cloning, Lumio Detection, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, R

S. cerevisiae strain MaV203 contains deletions in endogenous GAL4 and GAL80 genes for use with GAL4-based two-hybrid systemsStrain also has leu2 and trp1 mutations for selection of ProQuest bait and prey vectorsMaV203 contains three GAL4 inducible report

S. cerevisiae strain MaV203 contains deletions in endogenous GAL4 and GAL80 genes for use with GAL4-based two-hybrid systemsHas leu2 and trp1 mutations for selection of ProQuest bait and prey vectorsMaV203 contains three GAL4 inducible reporter genes, pr

Subphysiological levels of steroid compound are used to induce expression from very low basal to high induced levelsFormulated for use in GeneSwitch™ SystemLypholizedMammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis,

Using specifically designed att sites for recombinational cloning, clone two, three, or four DNA fragments into any Gateway Destination vector containing attR1 and attR2 sitesResulting expression clone is ready for downstream expression and analysis appl

Utilizes Ni-NTA nickel-chelating resin and is supplied with native and denaturing buffers for efficient purification of recombinant proteins under different conditionsHis-Tagged Protein Purification, Protein and Peptide Purification, Protein Sample Prepa

Provide topoisomerase-I activated pcDNA3.1/NT-GFP-TOPO vectorTaq-amplified DNA fragments are ligated into these vectors in easy 5-minute reaction on bench topVector is designed for high-level expression of transient or stable GFP fusion proteins in wide

The vector offers the strong, constitutive promoter from the Drosophila actin 5C geneSmall size (5.4 kb) to improve DNA yields and increase subcloning efficiencyC-terminal V5 epitope tag for rapid detection with Anti-V5 AntibodyC-terminal 6xHis tag for s

Invitrogen offers state-of-the-art Gateway destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway destination vectors have attR sites

The vector allows generation of an adenovirus containing the target gene where constitutive, high-level expression is driven by the CMV promoterHigh efficiency and rapid recombination cloningProduces high titer adenoviral stocksEfficient delivery of the