Terrific Broth (TB) is used with glycerol in cultivating recombinant strains of Escherichia coli. Terrific Broth II is a rich nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, protein expression. It is certified for plasmid and other bacterial cultures in liquid medium. Higher cell densities are achieved using Terrific Broth in comparison to traditional LB Broth.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
• Terrific broth is a nutritional medium mainly constituted by peptone, yeast extract, glycerol (as a carbon source), amino acids, and water-soluble vitamins
Application
• Terrific broth is a highly enriched medium used with glycerol to cultivate bacteria, including Escherichia coli and recombinant strains
• Terrific broth medium is commonly used for maintainance of bacterial strains, for cloning, and for protein expression
• It is a certified medium for plasmid and other bacterial cultures in a liquid medium
• A higher cell density is obtained with this media compared to traditional LB broth