TRIS-buffered saline (TBS, 10X), with 0.5% Tween 20

TRIS-buffered saline (TBS, 10X), with 0.5% Tween 20 is used as a wash buffer and for sample preparation in general immunoassay applications, Tween 20 reduces nonspecific binding and protein:protein interactions.TBS is often used for diluting secondary antibodies or streptavidin-AP conjugate. TBS-Tween 20 is often used for washing steps.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
TRIS-buffered saline (TBS, 10X), with 0.5% Tween 20 is used as a wash buffer and for sample preparation in general immunoassay applications, Tween 20 reduces nonspecific binding and protein:protein interactions.TBS is often used for diluting secondary antibodies or streptavidin-AP conjugate. TBS-Tween 20 is often used for washing steps.

Solubility
It is soluble in water.

Notes
250mM Tris, 27mM KCl, 1.37M NaCl, pH 7.4 with 0.5% Tween 20