DNA Vectors

The Antibody-Expressing Positive Control Vector is a mammalian expression control that expresses a complete, full-length rabbit IgG. It can be used as a positive expression control for transient expression systems such as the Expi293™ Expression System,

Adds control elements from bacterial tetracycline resistance operon to effectively repress and derepress transcription from strong mammalian promoter sequencesAllows tetracycline-regulated expression of gene of interest in mammalian host cells expressing

Displayed proteins can be analyzed for their ability to interact with known or putative ligandsProteins of interest are targeted and anchored to cell surface by cloning the gene of interest in frame with vectors unique N-terminal secretion signal and C-t

Competent cells can be frozen for future use, minimizing wasteCompetent cells can be prepared in less than 30 minutesCompetent cell preparation and transformation solutions are optimized for use with Pichia pastoris are provided ready to use and are test

Can be used as a positive expression control for transient expression systems such as the Expi293 Expression System, the ExpiCHO Expression System, the FreeStyle 293 Expression System, and the FreeStyle CHO Expression System. Features:A mix of pcDNA3.4 p

A subclone of the 293 cell line and has a relatively flat morphology, it facilitates the initial production, amplification and titering of replication-incompetent adenovirus.Flat morphology of cells makes titering procedure easier and suitable for screen

The BacMam pCMV-Dest Vector combines Gateway™ cloning and BacMam gene expression technologies for easy recombination-based cloning and baculovirus-based expression of a target gene in variety of cell types. With BacMam technology, a modified insect cell

Recombinant viruses have full-length, functional lacZ gene resulting in production of blue plaques for easy identification and purificationBac-N-Blue DNA can be used with any polyhedrin promoter-based baculovirus transfer vectorLinearized at three sites,

Relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cellsTime-saving expression bacmid: expression cassette of pFastBacvector recombines with parent bacmid in DH10BacE. c

Strong polyhedrin promoter for protein expressionThree reading frames for simplified cloningN-terminal 6 x His tag for simple purification of recombinant fusion proteinsTEV protease cleavage site for removal of histidine tag following protein purificatio

Contains pFastBac™1 vector, as well expression control vector, intended for use as part of Bac-to-Bac Baculovirus Expression System, which enables efficent production of recombinant baculovirus for expression testing in insect cellsRelies on generation o

Baculovirus Production and Titering, Cloning, Gateway Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Engineered to include attR sites for quick and efficient recombination with Gateway entry cloneGene of interest is recombined from entry clone into BaculoDirect Linear DNA using simple, one-hour LR reactionResulting reaction mix contains recombinant bacu

Destination vectors carrying polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteinspDEST™10 vector containing TEV protease cleavage site for removal of fusion tag afte

Based on T7 expression vectors originally developed by Studier and colleagues, high-level expression is achieved because T7 RNA polymerase is more processive than native E. coliRNA polymerase and is dedicated to transcription of gene of interestProtein p