No ligase, post-PCR procedures, or PCR primers containing specific sequences are requiredOnce cloned, analyzed, and transfected, PCR product will express directly in mammalian cell linespcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-le
No ligase, post-PCR procedures, or PCR primers containing specific sequences are requiredOnce cloned, analyzed, and transfected, PCR product will express directly in mammalian cell linespcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-le
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